| Autor: |
Maurice B, Hallett, Maha, Al-Jumaa, Sharon, Dewitt |
| Rok vydání: |
2019 |
| Předmět: |
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| Zdroj: |
Methods in molecular biology (Clifton, N.J.). 2087 |
| ISSN: |
1940-6029 |
| Popis: |
The measurement and manipulation of cytosolic free Ca(2+) of neutrophils is crucial for investigating the mechanisms within living neutrophils which generate Ca(2+) signals and the cellular responses triggered by them. Optical methods for this are the most applicable for neutrophils and are discussed here, especially the use of fluorescent indicators of Ca(2+) and photoactivation of reagents involved in Ca(2+) signaling. Both of these synthetic agents can be loaded into neutrophils as lipid-soluble esters or can be microinjected into the cell. In this chapter, we will outline some of the techniques that have been used to monitor, visualize, and manipulate Ca(2+) in neutrophils. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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