Use of differentiating embryonic stem cells in pharmacological studies

Autor: Wdziekonski, B., Villageois, P., Vernochet, C., Phillips, B., Christian Dani
Přispěvatelé: Institut de signalisation, biologie du développement et cancer ( ISBDC ), Université Nice Sophia Antipolis ( UNS ), Université Côte d'Azur ( UCA ) -Université Côte d'Azur ( UCA ) -Centre National de la Recherche Scientifique ( CNRS ), Institut de signalisation, biologie du développement et cancer (ISBDC), Centre National de la Recherche Scientifique (CNRS)-Université Nice Sophia Antipolis (... - 2019) (UNS), COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Université Côte d'Azur (UCA)
Jazyk: angličtina
Rok vydání: 2006
Předmět:
Cell Culture Techniques
Drug Evaluation
Preclinical

Gene Expression
MESH : Adipocytes
MESH: Base Sequence
Mice
Osteogenesis
MESH: Reverse Transcriptase Polymerase Chain Reaction
Adipocytes
MESH : Osteogenesis
MESH : RNA
MESH : Embryo
Mammalian

MESH: Animals
MESH : Drug Evaluation
Preclinical

MESH: Osteogenesis
[SDV.BDD]Life Sciences [q-bio]/Development Biology
Adipogenesis
Reverse Transcriptase Polymerase Chain Reaction
MESH : Adipogenesis
MESH : Reverse Transcriptase Polymerase Chain Reaction
Cell Differentiation
MESH : Pluripotent Stem Cells
MESH: Pluripotent Stem Cells
MESH: Drug Evaluation
Preclinical

MESH : DNA Primers
MESH : Cell Differentiation
Pluripotent Stem Cells
MESH: Cell Differentiation
MESH: DNA Primers
MESH : Cell Culture Techniques
MESH: Gene Expression
MESH: RNA
MESH : Mice
Animals
[SDV.BBM]Life Sciences [q-bio]/Biochemistry
Molecular Biology

[ SDV.BDD ] Life Sciences [q-bio]/Development Biology
MESH: Mice
[ SDV.BBM ] Life Sciences [q-bio]/Biochemistry
Molecular Biology

MESH: Adipocytes
MESH : Osteoblasts
DNA Primers
MESH: Osteoblasts
MESH: Cell Culture Techniques
Osteoblasts
Base Sequence
MESH: Embryo
Mammalian

Embryo
Mammalian

MESH : Gene Expression
RNA
MESH : Base Sequence
MESH : Animals
MESH: Adipogenesis
Zdroj: Methods in Molecular Biology (Clifton then Totova)
Methods in Molecular Biology (Clifton then Totova), Humana Press (Springer Imprint), 2006, 329, pp.341-51. 〈10.1385/1-59745-037-5:341〉
Methods in Molecular Biology
Methods in Molecular Biology, Humana Press/Springer Imprint, 2006, 329, pp.341-51. ⟨10.1385/1-59745-037-5:341⟩
Scopus-Elsevier
ISSN: 1064-3745
1940-6029
DOI: 10.1385/1-59745-037-5:341〉
Popis: International audience; Adipocytes and osteoblasts are derived from a common precursor cell. It has been proposed that the bone loss commonly seen during aging or in the pathology of osteoporosis might be partly caused by a deregulation of the normal balance between osteoblast and adipocyte differentiation. In vitro differentiation of mouse embryonic stem cells toward the adipogenic and the osteogenic lineages provides a powerful system for testing effects of compounds on the developmental switch between adipogenesis and osteogenesis and identification of pharmacological targets. In this chapter, an improved protocol to commit mouse embryonic stem cells into both lineages at a correct rate is detailed.
Databáze: OpenAIRE