Use of differentiating embryonic stem cells in pharmacological studies
Autor: | Wdziekonski, B., Villageois, P., Vernochet, C., Phillips, B., Christian Dani |
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Přispěvatelé: | Institut de signalisation, biologie du développement et cancer ( ISBDC ), Université Nice Sophia Antipolis ( UNS ), Université Côte d'Azur ( UCA ) -Université Côte d'Azur ( UCA ) -Centre National de la Recherche Scientifique ( CNRS ), Institut de signalisation, biologie du développement et cancer (ISBDC), Centre National de la Recherche Scientifique (CNRS)-Université Nice Sophia Antipolis (... - 2019) (UNS), COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Université Côte d'Azur (UCA) |
Jazyk: | angličtina |
Rok vydání: | 2006 |
Předmět: |
Cell Culture Techniques
Drug Evaluation Preclinical Gene Expression MESH : Adipocytes MESH: Base Sequence Mice Osteogenesis MESH: Reverse Transcriptase Polymerase Chain Reaction Adipocytes MESH : Osteogenesis MESH : RNA MESH : Embryo Mammalian MESH: Animals MESH : Drug Evaluation Preclinical MESH: Osteogenesis [SDV.BDD]Life Sciences [q-bio]/Development Biology Adipogenesis Reverse Transcriptase Polymerase Chain Reaction MESH : Adipogenesis MESH : Reverse Transcriptase Polymerase Chain Reaction Cell Differentiation MESH : Pluripotent Stem Cells MESH: Pluripotent Stem Cells MESH: Drug Evaluation Preclinical MESH : DNA Primers MESH : Cell Differentiation Pluripotent Stem Cells MESH: Cell Differentiation MESH: DNA Primers MESH : Cell Culture Techniques MESH: Gene Expression MESH: RNA MESH : Mice Animals [SDV.BBM]Life Sciences [q-bio]/Biochemistry Molecular Biology [ SDV.BDD ] Life Sciences [q-bio]/Development Biology MESH: Mice [ SDV.BBM ] Life Sciences [q-bio]/Biochemistry Molecular Biology MESH: Adipocytes MESH : Osteoblasts DNA Primers MESH: Osteoblasts MESH: Cell Culture Techniques Osteoblasts Base Sequence MESH: Embryo Mammalian Embryo Mammalian MESH : Gene Expression RNA MESH : Base Sequence MESH : Animals MESH: Adipogenesis |
Zdroj: | Methods in Molecular Biology (Clifton then Totova) Methods in Molecular Biology (Clifton then Totova), Humana Press (Springer Imprint), 2006, 329, pp.341-51. 〈10.1385/1-59745-037-5:341〉 Methods in Molecular Biology Methods in Molecular Biology, Humana Press/Springer Imprint, 2006, 329, pp.341-51. ⟨10.1385/1-59745-037-5:341⟩ Scopus-Elsevier |
ISSN: | 1064-3745 1940-6029 |
DOI: | 10.1385/1-59745-037-5:341〉 |
Popis: | International audience; Adipocytes and osteoblasts are derived from a common precursor cell. It has been proposed that the bone loss commonly seen during aging or in the pathology of osteoporosis might be partly caused by a deregulation of the normal balance between osteoblast and adipocyte differentiation. In vitro differentiation of mouse embryonic stem cells toward the adipogenic and the osteogenic lineages provides a powerful system for testing effects of compounds on the developmental switch between adipogenesis and osteogenesis and identification of pharmacological targets. In this chapter, an improved protocol to commit mouse embryonic stem cells into both lineages at a correct rate is detailed. |
Databáze: | OpenAIRE |
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