| Autor: |
Maiko, Okada, Shin-ichiro, Takezawa, Yoshihiro, Mezaki, Ikuko, Yamaoka, Ichiro, Takada, Hirochika, Kitagawa, Shigeaki, Kato |
| Jazyk: |
angličtina |
| Rok vydání: |
2008 |
| Předmět: |
|
| Popis: |
The female sex steroid hormone oestrogen stimulates both cell proliferation and cell differentiation in target tissues. These biological actions are mediated primarily through nuclear oestrogen receptors (ERs). The ligand-dependent transactivation of ERs requires several nuclear co-regulator complexes; however, the cell-cycle-dependent associations of these complexes are poorly understood. By using a synchronization system, we found that the transactivation function of ERalpha at G2/M was lowered. Biochemical approaches showed that ERalpha associated with two discrete classes of ATP-dependent chromatin-remodelling complex in a cell-cycle-dependent manner. The components of the NuRD-type complex were identified as G2/M-phase-specific ERalpha co-repressors. Thus, our results indicate that the transactivation function of ERalpha is cell-cycle dependent and is coupled with a cell-cycle-dependent association of chromatin-remodelling complexes. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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