Intestinal lamina propria TcRgammadelta+ lymphocytes selectively express IL-10 and IL-17
| Autor: | K E, Asigbetse, P A, Eigenmann, C P, Frossard |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2010 |
| Předmět: |
Receptors
Antigen T-Cell gamma-delta/*metabolism Mice Inbred C3H ddc:618 T-Lymphocytes/cytology/immunology/*metabolism T-Lymphocytes Intestinal Mucosa/cytology/*immunology/metabolism Interleukin-17 Forkhead Transcription Factors/biosynthesis Forkhead Transcription Factors Receptors Antigen T-Cell gamma-delta Lymphocyte Activation Antigens CD/biosynthesis Interleukin-10 Immunophenotyping Interleukin-10/*secretion Mice Interleukin-17/*secretion Antigens CD Animals Female Intestinal Mucosa Immunologic Memory |
| Zdroj: | Journal of Investigational Allergology and Clinical Immunology, Vol. 20, No 5 (2010) pp. 391-401 |
| ISSN: | 1018-9068 |
| Popis: | BACKGROUND: The characteristics and roles of gut lymphocytes have been only partly elucidated, in particular with regard to activation patterns. OBJECTIVES: To characterize lymphocytes from various parts of the gut and examine their activation pattern as a network. METHODS: Lymphocytes were isolated from the epithelium, the lamina propria, Peyer's patches, mesenteric lymph nodes, the spleen, and peripheral blood of naive mice. They were then characterized for T cell phenotype, T cell receptors (TcRs), activation markers, and cytokine production. RESULTS: The results showed a gradient of cells with an increasing proportion of TcRgammadelta+, CD8alphaalpha+ cells towards the gut lumen, with the highest number found in intraepithelial lymphocytes. These cells, together with lamina propria lymphocytes (LPLs) were also characterized by a memory-like phenotype (CD25- CD45RB(low) and CD44(high)) and CD69 expression. CD8+ TcRgammadelta+ LPLs produced IL-10 and IL-17, while TcRalphabeta+ LPLs were FoxP3 positive. CONCLUSIONS: Gut lymphocytes express various receptors and cytokines according to their location. These specific features suggest a differential function for gut lymphocytes depending on their location. |
| Databáze: | OpenAIRE |
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