Developmental adaptations of trypanosome motility to the tsetse fly host environments unravel a multifaceted in vivo microswimmer system
| Autor: | Schuster, Sarah, Krüger, Timothy, Subota, Ines, Thusek, Sina, Rotureau, Brice, Beilhack, Andreas, Engstler, Markus |
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| Přispěvatelé: | Biocenter University of Würzburg = Biozentrum der Universität Würzburg, Julius-Maximilians-Universität Würzburg [Wurtzbourg, Allemagne] (JMU), University Hospital of Würzburg, Biologie cellulaire des Trypanosomes - Trypanosome Cell Biology, Institut Pasteur [Paris]-Institut National de la Santé et de la Recherche Médicale (INSERM), Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut Pasteur [Paris], Julius-Maximilians-Universität Würzburg (JMU), Institut Pasteur [Paris] (IP)-Institut National de la Santé et de la Recherche Médicale (INSERM) |
| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
Trypanosoma
Tsetse Flies QH301-705.5 none Science sleeping sickness [SDV]Life Sciences [q-bio] Trypanosoma brucei brucei MESH: Locomotion MESH: Microscopy Fluorescence MESH: Host-Parasite Interactions Host-Parasite Interactions ddc:571 biophysics Animals structural biology MESH: Animals [SDV.MP.PAR]Life Sciences [q-bio]/Microbiology and Parasitology/Parasitology tsetse fly Biology (General) MESH: Tsetse Flies Animal Structures MESH: Trypanosoma brucei brucei microswimmer Biophysics and Structural Biology [SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology Microscopy Fluorescence Medicine MESH: Animal Structures Locomotion Research Article |
| Zdroj: | eLife eLife, eLife Sciences Publication, 2017, 6, ⟨10.7554/eLife.27656⟩ eLife, Vol 6 (2017) eLife, 2017, 6, ⟨10.7554/eLife.27656⟩ |
| ISSN: | 2050-084X |
| Popis: | The highly motile and versatile protozoan pathogen Trypanosoma brucei undergoes a complex life cycle in the tsetse fly. Here we introduce the host insect as an expedient model environment for microswimmer research, as it allows examination of microbial motion within a diversified, secluded and yet microscopically tractable space. During their week-long journey through the different microenvironments of the fly´s interior organs, the incessantly swimming trypanosomes cross various barriers and confined surroundings, with concurrently occurring major changes of parasite cell architecture. Multicolour light sheet fluorescence microscopy provided information about tsetse tissue topology with unprecedented resolution and allowed the first 3D analysis of the infection process. High-speed fluorescence microscopy illuminated the versatile behaviour of trypanosome developmental stages, ranging from solitary motion and near-wall swimming to collective motility in synchronised swarms and in confinement. We correlate the microenvironments and trypanosome morphologies to high-speed motility data, which paves the way for cross-disciplinary microswimmer research in a naturally evolved environment. DOI: http://dx.doi.org/10.7554/eLife.27656.001 |
| Databáze: | OpenAIRE |
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