Disruption of AtOCT1, an organic cation transporter gene, affects root development and carnitine-related responses in Arabidopsis
| Autor: | Christine, Lelandais-Brière, Mariana, Jovanovic, Gisèle A M, Torres, Yolande, Perrin, Rémi, Lemoine, Fabienne, Corre-Menguy, Caroline, Hartmann |
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| Přispěvatelé: | Institut des sciences du végétal (ISV), Centre National de la Recherche Scientifique (CNRS), Centro de Pesquisa e Desenvolvimento de Recursos Genéticos Vegetais, Instituto Agronômico de Campinas, Génie Enzymatique et Cellulaire (GEC), Université de Technologie de Compiègne (UTC)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS), Transport des assimilats (TA), Université de Poitiers-Centre National de la Recherche Scientifique (CNRS), Laboratoire de Physiologie et Biochimie Végétales, Centre National de la Recherche Scientifique (CNRS)-Université de Poitiers |
| Jazyk: | angličtina |
| Rok vydání: | 2007 |
| Předmět: |
MESH: Mutation
[SDV]Life Sciences [q-bio] Molecular Sequence Data Arabidopsis MESH: Plant Roots Saccharomyces cerevisiae MESH: Amino Acid Sequence Plant Roots Gene Expression Regulation Plant Carnitine [SDV.BV]Life Sciences [q-bio]/Vegetal Biology MESH: Arabidopsis MESH: Cloning Molecular Amino Acid Sequence Cloning Molecular MESH: Gene Expression Regulation Plant MESH: Phylogeny Phylogeny ComputingMilieux_MISCELLANEOUS MESH: Molecular Sequence Data Arabidopsis Proteins MESH: Carnitine Organic Cation Transporter 1 MESH: Organic Cation Transporter 1 MESH: Saccharomyces cerevisiae Mutation |
| Zdroj: | Plant Journal Plant Journal, Wiley, 2007, 51 (2), pp.154-64. ⟨10.1111/j.1365-313X.2007.03131.x⟩ Plant Journal, Wiley, 2007, 51, pp.154-164. ⟨10.1111/j.1365-313X.2007.03131.x⟩ |
| ISSN: | 0960-7412 1365-313X |
| DOI: | 10.1111/j.1365-313X.2007.03131.x⟩ |
| Popis: | In animals, organic cation/carnitine transporters (OCTs) are involved in homeostasis and distribution of various small endogenous amines (e.g. carnitine, choline) and detoxification of xenobiotics such as nicotine. Here, we describe the characterization of AtOCT1, an Arabidopsis protein that shares most of the conserved features of mammalian plasma membrane OCTs. Transient expression of an AtOCT1::GFP fusion protein in onion epidermal cells and Arabidopsis protoplasts supported localization in the plasmalemma. AtOCT1 functionally complemented the Deltacit2/Deltaagp2p yeast strain that is defective in plasma membrane carnitine transport. Disruption of AtOCT1 in an Arabidopsis oct1-1 knockout mutant affected both the expression of carnitine-related genes and the developmental defects induced by exogenous carnitine. RT-PCR and promoter-uidA fusion analysis showed that AtOCT1 was expressed in vascular tissues of various organs and at sites of lateral root formation. Correlating with this expression pattern, oct1-1 seedlings grown in vitro exhibited a higher degree of root branching than the wild-type, showing that the disruption of AtOCT1 affected root development under certain conditions. |
| Databáze: | OpenAIRE |
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