Surface proteins involved in the adhesion of Streptococcus salivarius to human intestinal epithelial cells
| Autor: | Chaffanel, Fanny, Charron-Bourgoin, Florence, Soligot, Claire, Kebouchi, Mounira, Bertin, Stéphane, Payot, Sophie, Le Roux, Yves, Leblond-Bourget, Nathalie |
|---|---|
| Přispěvatelé: | Dynamique des Génomes et Adaptation Microbienne (DynAMic), Université de Lorraine (UL)-Institut National de la Recherche Agronomique (INRA), Unité de Recherches Animal et Fonctionnalités des Produits Animaux (URAFPA), Institut National de la Recherche Agronomique (INRA)-Université de Lorraine (UL) |
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
Whole Genome Sequencing
Lacticaseibacillus rhamnosus Streptococcal surface proteins Membrane Proteins Epithelial Cells Streptococcus salivarius [SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology Bacterial Adhesion Applied Microbial and Cell Physiology Adhesion Humans HT29-MX Caco-2/TC7 Caco-2 Cells Adhesins Bacterial HT29 Cells Gene Deletion Genome Bacterial |
| Zdroj: | Applied Microbiology and Biotechnology Applied Microbiology and Biotechnology, Springer Verlag, 2018, 102 (6), pp.2851-2865. ⟨10.1007/s00253-018-8794-y⟩ |
| ISSN: | 0175-7598 1432-0614 |
| DOI: | 10.1007/s00253-018-8794-y⟩ |
| Popis: | The adhesion properties of 14 Streptococcus salivarius strains to mucus (HT29-MTX) and non-mucus secreting (Caco-2/TC7) human intestinal epithelial cells were investigated. Ability to adhere to these two eukaryotic cell lines greatly differs between strains. The presence of mucus played a major factor in adhesion, likely due to high adhesiveness to mucins present in the native human mucus layer covering the whole cell surface. Only one S. salivarius strain (F6-1), isolated from the feces of a healthy baby, was found to strongly adhere to HT-29 MTX cells at a level comparable to that of Lactobacillus rhamnosus GG, a probiotic strain considered to be highly adherent. By sequencing the genome of F6-1, we were able to identify 36 genes encoding putative surface proteins. Deletion mutants were constructed for six of them and their adhesion abilities on HT-29 MTX cells were checked. Our study confirmed that four of these genes encode adhesins involved in the adhesion of S. salivarius to host cells. Such adhesins were also identified in other S. salivarius strains. Electronic supplementary material The online version of this article (10.1007/s00253-018-8794-y) contains supplementary material, which is available to authorized users. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full text from SpringerLink