Interaction of ES cell derived neural progenitor cells with natural killer cells and cytotoxic T cells
| Autor: | Casimir, de Rham, Jean, Villard |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2013 |
| Předmět: |
Chromium
Time Factors Cell Communication/drug effects Killer Cells Natural/cytology Succinimides Interferon-gamma/secretion Cell Communication Cell Separation Lymphocyte Activation Neural Stem Cells/cytology/drug effects Fluoresceins/metabolism Interferon-gamma Neural Stem Cells Humans Embryonic Stem Cells reproductive and urinary physiology Culture Media/pharmacology ddc:616 Chromium/metabolism Staining and Labeling Succinimides/metabolism T-Lymphocytes Cytotoxic/cytology/drug effects Fluoresceins equipment and supplies Flow Cytometry Lymphocyte Activation/drug effects Culture Media Killer Cells Natural embryonic structures Embryonic Stem Cells/cytology/drug effects biological phenomena cell phenomena and immunity T-Lymphocytes Cytotoxic |
| Zdroj: | Methods in Molecular Biology, Vol. 1029 (2013) pp. 65-75 |
| ISSN: | 1064-3745 |
| Popis: | Knowing that human embryonic stem cells (HESC) can be derived into several different cells types render these cells very attractive to cure diseases. Unless these stem cells are originated from the patient itself, they will be isolated from a donor, who is genetically unrelated to the recipient. This situation will mimic an allogenic transplantation with an immune response against the transplanted cells. The immunogenicity of the HESC and the potential of NK and T-cells to target HESC and the lineage derived from HESC have to be addressed. Several different tests do exist to analyse NK cells and T-cells activity against HESC and its progenitor cells. In this chapter review the capacity of NK and T cells against neural progenitor derived from HESC, through a classical and a novel approach that combined the phenotype and also the functionality of the effector cells. In addition, we also demonstrate in the same test that we can determine the lysis of the progenitor cells by flow cytometry. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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