[The study of orthopoxvirus genes for Kelch-like proteins. II. Construction of cowpox virus variants with targeted gene deletions]

Autor:	Iv, Kolosova, Sv, Seregin, Gv, Kochneva, Ei, Riabchikova, Ev, Bessmel Tseva, In, Babkina, Te, Solenova, Igor Babkin, Sn, Shchelkunov
Rok vydání:	2003
Předmět:	Viral Proteins Mutation Animals Cowpox Chick Embryo Chorion Carrier Proteins Cowpox virus Virus Replication Cells Cultured Gene Deletion Plasmids
Zdroj:	Europe PubMed Central
ISSN:	0026-8984
Popis:	Integrative plasmids p delta C, p delta D, and p delta G were designed to contain a selective marker beyond the region of homology to virus DNA and to allow construction of recombinant cowpox viruses (CPV) that lack C18L, D11L, or G3L coding for kelch-like proteins. CPV mutants lacking one (C18L, D11L, or G3L), two (D11L/G3L or C18L/D11L), or three (D11L/G3L/C18L, that is, all) kelch-like protein genes of the left variable region of the virus genome were obtained. Impaired reproduction was observed for the triple mutant. Pocks produced by the triple mutant and the original virus differed in size and morphology. In addition, the two CPV variants differed in destructive changes caused in the chorioallantoic membrane of chicken embryos.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=pmid_dedup__::2d903431904b70de3cebc71864a3cfd3 https://pubmed.ncbi.nlm.nih.gov/12942630 Zobrazit plný text záznamu