Toll-like receptor 2/4 inhibitors can reduce preterm birth in mice
| Autor: | Xu Jing, Chen Min, Liu Qi Yun, Hu Shun Qin, Li Li Rui, Li Jia, Ma Run Mei |
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| Rok vydání: | 2020 |
| Předmět: |
Toll-like receptor4
Lipopolysaccharides Medicine (General) Tumor Necrosis Factor-alpha lipopolysaccharide interleukin-10 preterm birth Toll-like receptor2 Toll-Like Receptor 2 Pre-Clinical Research Report Toll-Like Receptor 4 Mice R5-920 Pregnancy Animals Premature Birth Female tumor necrosis factor-α |
| Zdroj: | The Journal of International Medical Research Journal of International Medical Research, Vol 48 (2020) |
| ISSN: | 1473-2300 |
| Popis: | Objectives Preterm birth (PTB) occurs in 5% to 18% of newborns. However, the underlying inflammatory mechanisms have not been elucidated. Methods We established a mouse model of infection-associated PTB. Physical signs in pregnant mice with or without lipopolysaccharide (LPS) treatment were observed, and the frequencies of Toll-like receptor (TLR)2- and TLR4-positive CD11b+ cells were analyzed. Cytokine levels in plasma and pathological changes were assessed following LPS treatment. A rescue experiment was used to probe potential immunologic mechanisms underlying PTB. Results Lymphocyte infiltration could be observed in the placentas of mice following intrauterine injection with LPS. The percentage of inflammatory cells decreased 12 hours after treatment. Moreover, TLR2 and TLR4 expression in peripheral blood cells was significantly increased 4 hours after intraperitoneal injection of LPS. Peak TLR2 and TLR4 expression in peripheral blood cells occurred 8 hours post-treatment. TLR4 and TLR-2/4 inhibitors reduced levels of interleukin-10, interferon-γ, and tumor necrosis factor-α in peripheral blood and delayed PTB. Conclusions TLR2 and TLR4 inhibition could play important roles in PTB. |
| Databáze: | OpenAIRE |
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