Heart regeneration in the Mexican cavefish
| Autor: | Stockdale, W.T., Lemieux, M.E., Killen, A.C., Zhao, J., Hu, Z., Riepsaame, J., Hamilton, N., Kudoh, T., Riley, P.R., van Aerle, R., Yamamoto, Y., Mommersteeg, M.T.M. |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
lrrc10
Wound Healing heart regeneration QTL Characidae myocardial proliferation Myocardium Quantitative Trait Loci Heart Zebrafish Proteins Article Up-Regulation Kinetics lcsh:Biology (General) Mutation Animals Regeneration Mexican cavefish Myocytes Cardiac fibrotic scar lcsh:QH301-705.5 Zebrafish Cell Proliferation |
| Zdroj: | Cell Reports, Vol 25, Iss 8, Pp 1997-2007.e7 (2018) Cell Reports BASE-Bielefeld Academic Search Engine |
| ISSN: | 2211-1247 |
| Popis: | Summary Although Astyanax mexicanus surface fish regenerate their hearts after injury, their Pachón cave-dwelling counterparts cannot and, instead, form a permanent fibrotic scar, similar to the human heart. Myocardial proliferation peaks at similar levels in both surface fish and Pachón 1 week after injury. However, in Pachón, this peak coincides with a strong scarring and immune response, and ultimately, cavefish cardiomyocytes fail to replace the scar. We identified lrrc10 to be upregulated in surface fish compared with Pachón after injury. Similar to cavefish, knockout of lrrc10 in zebrafish impairs heart regeneration without affecting wound cardiomyocyte proliferation. Furthermore, using quantitative trait locus (QTL) analysis, we have linked the degree of heart regeneration to three loci in the genome, identifying candidate genes fundamental to the difference between scarring and regeneration. Our study provides evidence that successful heart regeneration entails a delicate interplay between cardiomyocyte proliferation and scarring. Graphical Abstract Highlights • Astyanax mexicanus surface fish regenerate their hearts, but Pachón cavefish cannot • Successful regeneration is a delicate interplay between proliferation and scarring • lrrc10 is required for heart regeneration • Using QTL analysis, we have identified three genomic loci linked to heart regeneration Stockdale et al. identify the Mexican cavefish as a model for heart regeneration research. This model provides an opportunity for direct comparison of natural regeneration versus scarring within a single species. Furthermore, they have identified key genomic regions regulating heart regeneration using an unbiased quantitative trait loci analysis approach. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|