Design and Generation of Gene-Targeting Vectors

Autor:	Wefers, Benedikt, Wurst, Wolfgang, Kühn, Ralf
Rok vydání:	2015
Předmět:	EUCOMM/KOMP ddc:590 conditional KO mice targeting vector design knockout mice gene targeting
Zdroj:	Current protocols in mouse biology 1(1), 199-211 (2011). doi:10.1002/9780470942390.mo100179
ISSN:	2161-2617
DOI:	10.1002/9780470942390.mo100179
Popis:	This unit provides an overview of the major types of mutant alleles that can be generated by gene targeting in ES cells. It presents the growing public resources of premade gene targeting vectors, modified ES cells, and mutant mice. General guidelines for the design of targeting vectors are followed by protocols for the construction of vectors to generate knockout (KO), conditional KO, and subtle mutant alleles. Curr. Protoc. Mouse Biol. 1:199-211. © 2011 by John Wiley & Sons, Inc.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=pmid_dedup__::2c132ae0d5fb23fc7f43351b7f4a94ee https://pubmed.ncbi.nlm.nih.gov/26068993 Zobrazit plný text záznamu Plný text