Somatostatin down-regulates the expression and release of endozepines from cultured rat astrocytes via distinct receptor subtypes

Autor: Masmoudi, Olfa, Gandolfo, Pierrick, Tokay, Tursonjan, Leprince, Jérôme, Ravni, Aurélia, Vaudry, Hubert, Tonon, Marie-Christine
Přispěvatelé: Neuroendocrinologie cellulaire et moléculaire, Université de Rouen Normandie (UNIROUEN), Normandie Université (NU)-Normandie Université (NU)-Institut National de la Santé et de la Recherche Médicale (INSERM), Institut Fédératif de Recherches Multidisciplinaires sur les Peptides (IFRMP 23), Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre de Lutte Contre le Cancer Henri Becquerel Normandie Rouen (CLCC Henri Becquerel)-Université de Rouen Normandie (UNIROUEN), Normandie Université (NU)-Normandie Université (NU)-Université Le Havre Normandie (ULH), Normandie Université (NU)-CHU Rouen, Normandie Université (NU)-Centre National de la Recherche Scientifique (CNRS)
Rok vydání: 2005
Předmět:
Time Factors
MESH: Somatostatin
MESH: Drug Interactions
[SDV.NEU.NB]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]/Neurobiology
[CHIM.THER]Chemical Sciences/Medicinal Chemistry
MESH: Neuropeptides
MESH: Animals
Newborn
MESH: Down-Regulation
MESH: Dose-Response Relationship
Drug
MESH: Reverse Transcriptase Polymerase Chain Reaction
[SDV.BC.IC]Life Sciences [q-bio]/Cellular Biology/Cell Behavior [q-bio.CB]
Cyclic AMP
MESH: Phenanthrolines
MESH: Animals
Drug Interactions
Receptors
Somatostatin
Enzyme Inhibitors
MESH: Peptide Fragments
MESH: Cyclic AMP
Cells
Cultured
Chromatography
High Pressure Liquid
Diazepam Binding Inhibitor
Reverse Transcriptase Polymerase Chain Reaction
Brain
MESH: Enzyme Inhibitors
Somatostatin
hormones
hormone substitutes
and hormone antagonists
MESH: Cells
Cultured
Phenanthrolines
MESH: Colforsin
endocrine system
MESH: Rats
Radioimmunoassay
Down-Regulation
MESH: Radioimmunoassay
MESH: Brain
MESH: Analysis of Variance
MESH: Receptors
Somatostatin
MESH: Blotting
Northern
Animals
RNA
Messenger
Rats
Wistar
MESH: Chromatography
High Pressure Liquid
MESH: RNA
Messenger
Analysis of Variance
Dose-Response Relationship
Drug
MESH: Time Factors
Colforsin
Neuropeptides
MESH: Rats
Wistar
Blotting
Northern
Peptide Fragments
Rats
MESH: Astrocytes
Animals
Newborn
Astrocytes
[SDV.SP.PHARMA]Life Sciences [q-bio]/Pharmaceutical sciences/Pharmacology
MESH: Diazepam Binding Inhibitor
Zdroj: Journal of Neurochemistry
Journal of Neurochemistry, Wiley, 2005, 94 (3), pp.561-571. ⟨10.1111/j.1471-4159.2005.03076.x⟩
ISSN: 0022-3042
1471-4159
DOI: 10.1111/j.1471-4159.2005.03076.x⟩
Popis: International audience; Endozepines, a family of regulatory peptides related to diazepam-binding inhibitor (DBI), are synthesized and released by astroglial cells. Because rat astrocytes express various subtypes of somatostatin receptors (sst), we have investigated the effect of somatostatin on DBI mRNA level and endozepine secretion in rat astrocytes in secondary culture. Somatostatin reduced in a concentration-dependent manner the level of DBI mRNA in cultured astrocytes. This inhibitory effect was mimicked by the selective sst4 receptor agonist L803-087 but not by the selective sst1, sst2 and sst3 receptor agonists L779-591, L779-976 and L797-778, respectively. Somatostatin was unable to further reduce DBI mRNA level in the presence of the MEK inhibitor U0126. Somatostatin and the sst1, sst2 and sst4 receptor agonists induced a concentration-dependent inhibition of endozepine release. Somatostatin and the sst1, sst2 and sst4 receptor agonists also inhibited cAMP formation dose-dependently. In addition, somatostatin reduced forskolin-induced endozepine release. H89 mimicked the inhibitory effect of somatostatin on endozepine secretion. In contrast the PLC inhibitor U73122, the PKC activator PMA and the PKC inhibitor calphostin C had no effect on somatostatin-induced inhibition of endozepine release. The present data demonstrate that somatostatin reduces DBI mRNA level mainly through activation of sst4 receptors negatively coupled to the MAPK pathway, and inhibits endozepine release through activation of sst1, sst2 and sst4 receptors negatively coupled to the adenylyl cyclase/PKA pathway.
Databáze: OpenAIRE
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