Acute exposure to silica nanoparticles enhances mortality and increases lung permeability in a mouse model of Pseudomonas aeruginosa pneumonia

Autor: Delaval, Mathilde, Boland, Sonja, Solhonne, Brigitte, Nicola, Marie-Anne, Mornet, Stéphane, Baeza-Squiban, Armelle, Sallenave, Jean-Michel, Garcia-Verdugo, Ignacio
Přispěvatelé: Unité de Biologie Fonctionnelle et Adaptative (BFA (UMR_8251 / U1133)), Université Paris Diderot - Paris 7 (UPD7)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Défense innée et inflammation, Institut Pasteur [Paris]-Institut National de la Santé et de la Recherche Médicale (INSERM), Physiopathologie et Epidémiologie des Maladies Respiratoires (PHERE (UMR_S_1152 / U1152)), Université Paris Diderot - Paris 7 (UPD7)-Institut National de la Santé et de la Recherche Médicale (INSERM), Imagerie Dynamique (Plate-Forme) (PFID), Institut Pasteur [Paris], Institut de Chimie de la Matière Condensée de Bordeaux (ICMCB), Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Institut Polytechnique de Bordeaux-Université de Bordeaux (UB), Cellule Pasteur, Université Paris Diderot - Paris 7 (UPD7)-PRES Sorbonne Paris Cité, This work was supported by the INSERM, Université Paris 7 Diderot and ANSES EST 2010/2/79 project, Institut Pasteur [Paris] (IP)-Institut National de la Santé et de la Recherche Médicale (INSERM), Institut Pasteur [Paris] (IP), Université de Bordeaux (UB)-Institut Polytechnique de Bordeaux-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), BMC, BMC
Jazyk: angličtina
Rok vydání: 2014
Předmět:
Male
Alveolar macrophages
MESH: Macrophages
Alveolar
MESH: Pulmonary Alveoli
Health
Toxicology and Mutagenesis
[SDV]Life Sciences [q-bio]
Toxicology
MESH: Animals
MESH: Phagocytosis
Lung
Inhalation Exposure
Alveolar permeability
MESH: Cytokines
MESH: Pseudomonas Infections
respiratory system
MESH: Immunoglobulin M
[SDV] Life Sciences [q-bio]
MESH: Survival Analysis
Pseudomonas aeruginosa
MESH: Pseudomonas aeruginosa
Cytokines
MESH: Inhalation Exposure
SiO2
Infection
Bronchoalveolar Lavage Fluid
MESH: Pneumonia
Bacterial
Surface Properties
Capillary Permeability
Phagocytosis
MESH: Mice
Inbred C57BL
Selenium Oxides
Pseudomonas
Macrophages
Alveolar
Pneumonia
Bacterial
Animals
Pseudomonas Infections
MESH: Particle Size
Particle Size
Inflammation
MESH: Surface Properties
MESH: Bronchoalveolar Lavage Fluid
Research
MESH: Capillary Permeability
MESH: Selenium Oxides
Survival Analysis
MESH: Male
Mice
Inbred C57BL
Pulmonary Alveoli
Immunoglobulin M
Nanoparticles
MESH: Nanoparticles
Zdroj: Particle and Fibre Toxicology
Particle and Fibre Toxicology, BioMed Central, 2014, 12 (1), pp.1. ⟨10.1186/s12989-014-0078-9⟩
Particle and Fibre Toxicology, 2014, 12 (1), pp.1. ⟨10.1186/s12989-014-0078-9⟩
ISSN: 1743-8977
DOI: 10.1186/s12989-014-0078-9⟩
Popis: Background The lung epithelium constitutes the first barrier against invading pathogens and also a major surface potentially exposed to nanoparticles. In order to ensure and preserve lung epithelial barrier function, the alveolar compartment possesses local defence mechanisms that are able to control bacterial infection. For instance, alveolar macrophages are professional phagocytic cells that engulf bacteria and environmental contaminants (including nanoparticles) and secrete pro-inflammatory cytokines to effectively eliminate the invading bacteria/contaminants. The consequences of nanoparticle exposure in the context of lung infection have not been studied in detail. Previous reports have shown that sequential lung exposure to nanoparticles and bacteria may impair bacterial clearance resulting in increased lung bacterial loads, associated with a reduction in the phagocytic capacity of alveolar macrophages. Results Here we have studied the consequences of SiO2 nanoparticle exposure on Pseudomonas aeruginosa clearance, Pseudomonas aeruginosa-induced inflammation and lung injury in a mouse model of acute pneumonia. We observed that pre-exposure to SiO2 nanoparticles increased mice susceptibility to lethal pneumonia but did not modify lung clearance of a bioluminescent Pseudomonas aeruginosa strain. Furthermore, internalisation of SiO2 nanoparticles by primary alveolar macrophages did not reduce the capacity of the cells to clear Pseudomonas aeruginosa. In our murine model, SiO2 nanoparticle pre-exposure preferentially enhanced Pseudomonas aeruginosa-induced lung permeability (the latter assessed by the measurement of alveolar albumin and IgM concentrations) rather than contributing to Pseudomonas aeruginosa-induced lung inflammation (as measured by leukocyte recruitment and cytokine concentration in the alveolar compartment). Conclusions We show that pre-exposure to SiO2 nanoparticles increases mice susceptibility to lethal pneumonia but independently of macrophage phagocytic function. The deleterious effects of SiO2 nanoparticle exposure during Pseudomonas aeruginosa-induced pneumonia are related to alterations of the alveolar-capillary barrier rather than to modulation of the inflammatory responses. Electronic supplementary material The online version of this article (doi:10.1186/s12989-014-0078-9) contains supplementary material, which is available to authorized users.
Databáze: OpenAIRE
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