Regulatory T cells may participate in Helicobacter pylori persistence in gastric MALT lymphoma: lessons from an animal model
| Autor: | Laur, Amandine Marine, Floch, Pauline, Chambonnier, Lucie, Benejat, Lucie, Korolik, Victoria, Giese, Alban, Dubus, Pierre, Mégraud, Francis, Bandeira, Antonio, Lehours, Philippe |
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| Přispěvatelé: | Infection à helicobacter, inflammation et cancer, Université Bordeaux Segalen - Bordeaux 2-Institut National de la Santé et de la Recherche Médicale (INSERM), Laboratoire de bactériologie, CHU Bordeaux [Bordeaux], insitute for glycomics, Griffith University [Brisbane], Histologie et Pathologie Moléculaire, Université Bordeaux Segalen - Bordeaux 2, Biologie des Populations Lymphocytaires, Centre National de la Recherche Scientifique (CNRS)-Institut Pasteur [Paris], Alban Giese and the Experimental Histology unit were funded by the Cancéropôle Grand Sud Ouest, Institut Pasteur [Paris] (IP)-Centre National de la Recherche Scientifique (CNRS) |
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
regulatory T cell
Real-Time Polymerase Chain Reaction T-Lymphocytes Regulatory Helicobacter Infections Immunoenzyme Techniques Mice MESH: Lymphoma Non-Hodgkin Stomach Neoplasms MESH: Reverse Transcriptase Polymerase Chain Reaction Animals MESH: Animals RNA Messenger MALT lymphoma MESH: Immunoenzyme Techniques MESH: Lymphoma B-Cell Marginal Zone MESH: Mice MESH: RNA Messenger Helicobacter pylori Reverse Transcriptase Polymerase Chain Reaction MESH: Real-Time Polymerase Chain Reaction Lymphoma Non-Hodgkin animal model MESH: Helicobacter Infections MESH: T-Lymphocytes Regulatory Lymphoma B-Cell Marginal Zone MESH: Stomach Neoplasms Disease Models Animal MESH: Gastric Mucosa Gastric Mucosa MESH: Helicobacter pylori [SDV.IMM]Life Sciences [q-bio]/Immunology MESH: Disease Models Animal Research Paper |
| Zdroj: | Oncotarget Oncotarget, Impact journals, 2016, pp.3394-402. ⟨10.18632/oncotarget.6492⟩ Oncotarget, 2016, pp.3394-402. ⟨10.18632/oncotarget.6492⟩ |
| ISSN: | 1949-2553 |
| DOI: | 10.18632/oncotarget.6492⟩ |
| Popis: | International audience; It has been postulated that the emergence of autoimmune gastritis in neonatal thymectomised (d3Tx) BALB/c mice may be a consequence of post-surgery deficit in Tregs. In this study, previously obtained samples from d3Tx mice were used in order to determine whether thymectomy creates a deficit in this T cell subset thereby allowing the emergence of autoimmune phenomena as a prerequisite for GML. The splenic Treg reserve and the local recruitment of these cells in the gastric mucosa were investigated using complementary molecular and immunohistochemistry approaches. Higher Foxp3/CD3 ratios were found in the spleen of non-infected d3Tx mice compared to non-thymectomised (NTx) controls. These results indicate a relative enrichment of Tregs following thymectomy in adult mice. The absence of Treg depletion in d3Tx mice is in line with the absence of auto-immune gastritis in non-infected d3Tx mice. Higher levels of T cell and Treg infiltration were also found in the stomach of GML-developing d3Tx mice versus NTx mice. Surprisingly, inflammatory scores inversely correlated with the bacterial inoculum. The presence of a small Treg containing compartment among gastric biopsies of GML developing d3Tx mice may play a role in perseverance of a minimal bacterial numbers thereby maintaining an antigen-dependent stimulation and proliferation. |
| Databáze: | OpenAIRE |
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