Methods for the Administration of EDAR Pathway Modulators in Mice
| Autor: | Sonia, Schuepbach-Mallepell, Christine, Kowalczyk-Quintas, Angela, Dick, Mahya, Eslami, Michele, Vigolo, Denis J, Headon, Michael, Cheeseman, Holm, Schneider, Pascal, Schneider |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
integumentary system
Edar Receptor Drug Administration Routes Recombinant Proteins Disease Models Animal Mice Phenotype Treatment Outcome Animals Newborn stomatognathic system Ectodermal Dysplasia Animals Ectodermal Dysplasia/drug therapy Ectodermal Dysplasia/genetics Ectodermal Dysplasia/metabolism Edar Receptor/genetics Edar Receptor/metabolism Recombinant Proteins/administration & dosage Signal Transduction/drug effects Amniotic fluid EDAR signaling Ectodermal dysplasia Protein replacement therapy Route of administration Signal Transduction |
| Zdroj: | Methods in molecular biology, vol. 2248, pp. 167-183 |
| Popis: | Genetic deficiency of ectodysplasin A (EDA) causes X-linked hypohidrotic ectodermal dysplasia, a congenital condition characterized by the absence or abnormal formation of sweat glands, teeth, and several skin appendages. Stimulation of the EDA receptor (EDAR) with agonists in the form of recombinant EDA or anti-EDAR antibodies can compensate for the absence of Eda in a mouse model of Eda deficiency, provided that agonists are administered in a timely manner during fetal development. Here we provide detailed protocols for the administration of EDAR agonists or antagonists, or other proteins, by the intravenous, intraperitoneal, and intra-amniotic routes as well as protocols to collect blood, to visualize sweat gland function, and to prepare skulls in mice. |
| Databáze: | OpenAIRE |
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