Connective tissue progenitor cell growth characteristics on textured substrates
| Autor: | Alvaro Mata, Boehm, C., Fleischman, A. J., Muschler, G. F., Roy, S. |
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| Jazyk: | angličtina |
| Rok vydání: | 2007 |
| Předmět: |
Surface Properties
Cell Line PDMS stem cells Osteogenesis bone grafts Materials Testing Animals Humans Nanotechnology polydimethylsiloxane Dimethylpolysiloxanes Nanoscience & Nanotechnology Particle Size Original Research Cell Proliferation Osteoblasts Tissue Engineering Mesenchymal Stromal Cells Mesenchymal Stem Cells Cell Differentiation Pharmacology and Pharmaceutical Sciences Nanostructures MEMS Nylons Bone Substitutes microelectromechanical systems connective tissue progenitor cells osteoprogenitors |
| Zdroj: | Mata, A; Boehm, C; Fleischman, AJ; Muschler, GF; & Roy, S. (2007). Connective tissue progenitor cell growth characteristics on textured substrates.. International journal of nanomedicine, 2(3), 389-406. UC San Francisco: Retrieved from: http://www.escholarship.org/uc/item/8rk1p3hr International journal of nanomedicine, vol 2, iss 3 International Journal of Nanomedicine ResearcherID Scopus-Elsevier |
| Popis: | Growth characteristics of human connective tissue progenitor (CTP) cells were investigated on smooth and textured substrates, which were produced using MEMS (microelectromechanical systems) fabrication technology. Human bone marrow derived cells were cultured for 9 days under conditions promoting osteoblastic differentiation on polydimethylsiloxane (PDMS) substrates comprising smooth (non-patterned) surfaces (SMOOTH), 4 different cylindrical post micro-textures (POSTS) that were 7-10 microm high and 5, 10, 20, and 40 microm diameter, respectively, and channel micro-textures (CHANNELS) with curved cross-sections that were 11 microm high, 45 microm wide, and separated by 5 microm wide ridges. Standard glass-tissue culture surfaces were used as controls. Micro-textures resulted in the modification of CTP morphology, attachment, migration, and proliferation characteristics. Specifically, cells on POSTS exhibited more contoured morphology with closely packed cytoskeletal actin microfilaments compared to the more random orientation in cells grown on SMOOTH. CTP colonies on 10 gm-diameter POSTS exhibited higher cell number than any other POSTS, and a significant increase in cell number (442%) compared to colonies on SMOOTH (71%). On CHANNELS, colonies tended to be denser (229%) than on POSTS (up to 140% on 10 microm POSTS), and significantly more so compared to those on SMOOTH (104%). |
| Databáze: | OpenAIRE |
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