Over-expression of an Na+-and K+-permeable HKT transporter in barley improves salt tolerance
| Autor: | Mian, Afaq, Oomen, Ronald J F J, Isayenkov, Stanislav, Sentenac, Hervé, Maathuis, Frans J M, Véry, Anne-Aliénor |
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| Přispěvatelé: | Department of Biology [York], University of York [York, UK], Biochimie et Physiologie Moléculaire des Plantes (BPMP), Université de Montpellier (UM)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro)-Institut National de la Recherche Agronomique (INRA)-Centre National de la Recherche Scientifique (CNRS), Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Institut National de la Recherche Agronomique (INRA)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro), Biotechnology and Biological Sciences Research Council/Institut National de la Recherche Agronomique, Languedoc-Roussillon/China Arcus programme |
| Jazyk: | angličtina |
| Rok vydání: | 2011 |
| Předmět: |
salt tolerance
includer Xenopus Sodium food and beverages barley Hordeum Salt-Tolerant Plants Sodium Chloride Plants Genetically Modified HKT Plant Roots Membrane Potentials symport Gene Expression Regulation Plant Xylem Oocytes Potassium Animals [SDV.BV]Life Sciences [q-bio]/Vegetal Biology Cloning Molecular Cation Transport Proteins Plant Shoots Plant Proteins overexpression |
| Zdroj: | Plant Journal Plant Journal, Wiley, 2011, 68 (3), pp.468-79. ⟨10.1111/j.1365-313X.2011.04701.x⟩ |
| ISSN: | 0960-7412 1365-313X |
| DOI: | 10.1111/j.1365-313X.2011.04701.x⟩ |
| Popis: | International audience; Soil salinity is an increasing menace that affects agriculture across the globe. Plant adaptation to high salt concentrations involves integrated functions, including control of Na+ uptake, translocation and compartmentalization. Na+ transporters belonging to the HKT family have been shown to be involved in tolerance to mild salt stress in glycophytes such as Arabidopsis, wheat and rice by contributing to Na+ exclusion from aerial tissues. Here, we have analysed the role of the HKT transporter HKT2;1, which is permeable to K+ and Na+, in barley, a relatively salt-tolerant crop that displays a salt-including behaviour. In Xenopus oocytes, HvHKT2;1 co-transports Na+ and K+ over a large range of concentrations, displaying low affinity for Na+, variable affinity for K+ depending on external Na+ concentration, and inhibition by K+ (K(i) approximately 5 mm). HvHKT2;1 is predominantly expressed in the root cortex. Transcript levels are up-regulated in both roots and shoots by low K+ growth conditions, and in shoots by high Na+ growth conditions. Over-expression of HvHKT2;1 led to enhanced Na+ uptake, higher Na+ concentrations in the xylem sap, and enhanced translocation of Na+ to leaves when plants were grown in the presence of 50 or 100 mm NaCl. Interestingly, these responses were correlated with increased barley salt tolerance. This suggests that one of the factors that limits barley salt tolerance is the capacity to translocate Na+ to the shoot rather than accumulation or compartmentalization of this cation in leaf tissues. Thus, over-expression of HvHKT2;1 leads to increased salt tolerance by reinforcing the salt-including behaviour of barley. |
| Databáze: | OpenAIRE |
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