Aeromonas hydrophila flagella glycosylation: involvement of a lipid carrier

Autor: Merino Montero, Susana, Fulton, Kelly M., Twine, S.M., Wilhelms, Markus, Molero Andrade, Raquel, Tomàs Magaña, Juan
Přispěvatelé: Universitat de Barcelona
Jazyk: angličtina
Rok vydání: 2014
Předmět:
Bacterial Diseases
Motilitat cel·lular
Acetylgalactosamine
polymerase chain reaction
Glycobiology
Gene Expression
lcsh:Medicine
DNA fragmentation
Cell motility
Biochemistry
flagellin
Bacteris
protein purification
Carbohydrate Conformation
Gram Negative
lcsh:Science
Immune Response
Enzyme Classes
lipopolysaccharide
Enzymes
Bacterial Pathogens
Aeromonas hydrophila
Infectious Diseases
Carbohydrate Sequence
Medical Microbiology
Flagella
sequence alignment
Bacteris patògens
microscopy
Medicine
lipids (amino acids
peptides
and proteins)
Research Article
glycosylation
Immunology
Molecular Sequence Data
Biophysics
cell motility
ubiquitination
Microbiology
protein modification
epimerization
plasmid
bacterium isolation
tandem mass spectrometry
Regulació genètica
Amino Acid Sequence
enzyme specificity
Biology
Genetic regulation
Bacteria
ferredoxin nitrite reductase
lcsh:R
Glycosyltransferases
Bacteriology
molecular weight
nucleotide sequence
Flagellar Motility
bacterial strain
Lipid Metabolism
carbohydrates (lipids)
protein analysis
Pathogenic bacteria
bacteria
lcsh:Q
Glycolipids
biosynthesis
molecular model
Bacterial Biofilms
Carrier Proteins
Protein Processing
Post-Translational
Zdroj: PLoS ONE, Vol 9, Iss 2, p e89630 (2014)
PLoS ONE
Dipòsit Digital de la UB
Universidad de Barcelona
Recercat. Dipósit de la Recerca de Catalunya
instname
Popis: Polar flagellin proteins from Aeromonas hydrophila strain AH-3 (serotype O34) were found to be O-glycosylated with a heterogeneous glycan. Mutants unable to produce WecP or Gne enzymes showed altered motility, and the study of their polar flagellin glycosylation showed that the patterns of glycosylation differed from that observed with wild type polar flagellin. This suggested the involvement of a lipid carrier in glycosylation. A gene coding for an enzyme linking sugar to a lipid carrier was identified in strain AH-3 (WecX) and subsequent mutation abolished completely motility, flagella production by EM, and flagellin glycosylation. This is the first report of a lipid carrier involved in flagella O-glycosylation. A molecular model has been proposed. The results obtained suggested that the N -acetylhexosamines are N-acetylgalactosamines and that the heptasaccharide is completely independent of the O34-antigen lipopolysaccharide. Furthermore, by comparing the mutants with differing degrees of polar flagellin glycosylation, we established their importance in A. hydrophila flagella formation and motility.
Databáze: OpenAIRE
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