Nuclear location of synthetic oligonucleotides microinjected somatic cells: its implication in an antisense strategy

Autor:	mechti, nadir, Leonetti, J, Clarenc, J, Degols, G, Lebleu, B.
Přispěvatelé:	Institut de Recherche en Infectiologie de Montpellier (IRIM), Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS), Institut de Génétique Moléculaire de Montpellier (IGMM), Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM)
Jazyk:	angličtina
Rok vydání:	1991
Předmět:	Cell Nucleus Microinjections RNA Small Nuclear [SDV]Life Sciences [q-bio] Oligonucleotides Animals RNA Messenger Fibroblasts Oligonucleotides Antisense Ribonucleoproteins Small Nuclear Culture Media Serum-Free Vesicular stomatitis Indiana virus
Zdroj:	Nucleic Acids Symposium Series Nucleic Acids Symposium Series, Oxford University Press, 1991, pp.147-50
ISSN:	0261-3166 1746-8272
Popis:	International audience; Synthetic oligonucleotides are widely used to modulate gene expression. However their development as genetic tools and as molecule of therapeutic interest is restricted by the poor knowledge of their mechanism of action and of their uptake by cells. We recently found that microinjected oligonucleotides accumulates in the nucleus of the cells. These observations are described and their implications are discussed.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=pmid_dedup__::26589a9a85e84ce26c08b8bb5403c6be https://hal.archives-ouvertes.fr/hal-02361101 Zobrazit plný text záznamu