CD45RO+ memory T-cells produce IL-17 in patients with atherosclerosis
| Autor: | Behnamfar N, Mohammadjavad Zibaeenezhad, Golmoghaddam H, Doroudchi M |
|---|---|
| Rok vydání: | 2015 |
| Předmět: |
Adult
Male Systole Interleukin-17 Programmed Cell Death 1 Receptor Blood Pressure Cholesterol LDL Middle Aged Th1 Cells Atherosclerosis Flow Cytometry Immunophenotyping Interferon-gamma Gene Expression Regulation Diastole Case-Control Studies CD4 Antigens Humans Leukocyte Common Antigens Th17 Cells Female Immunologic Memory Triglycerides Signal Transduction |
| Zdroj: | Europe PubMed Central |
| ISSN: | 1165-158X |
| Popis: | Several CD4+ T helper (Th) cell subsets are shown to play a role in atherosclerotic lesion formation and progression. We investigated the frequencies of IL-17 and IFN-γ producing CD4+ T-cell subsets in the peripheral blood mononuclear cells (PBMCs) of 10 patients with atherosclerosis and 6 individuals with normal/insignificant coronary artery disease. Th1 and Th17 memory and effector T-cells were enumerated by flowcytometry and correlated with the clinical data and lipid profiles of the subjects. We found the ex-vivo (P=0.0001) and in-vitro production of IL-17 (P=0.001) but not IFN-γ by CD4+ memory T-cells of patients. CD45RO+ memory cells were the major producers of IL-17 and the CD4+CD45RO+PD-1- T-cells of the patients produced higher levels of IFN-γ than controls (P=0.02). Positive correlations between the frequency of CD4+CD45RO+IL-17+IFN-γ- T-cells and serum LDL-C (P=0.007), triglyceride (P=0.02), and systolic (P=0.001) and diastolic (P=0.009) blood pressures (BP) were found. The frequency of CD4+CD45RO+IL-17-IFN-γ- T-cells, which was higher in controls than patients, showed negative correlations with the serum LDL-C (P=0.01) and triglyceride (P=0.02) levels and systolic (P=0.003) and diastolic (P=0.01) BPs. The ex-vivo Th17 deviation of memory T-cells in atherosclerosis and high PD-1 expression are associated with the correlates of atherogenesis such as LDL, TG, and BP. |
| Databáze: | OpenAIRE |
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