Active transforming growth factor-β2 in the aqueous humor of posterior polymorphous corneal dystrophy patients
| Autor: | Stadnikova, Andrea, Dudakova, Lubica, Skalicka, Pavlina, Valenta, Zdenek, Filipec, Martin, Jirsova, Katerina |
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| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
Male
Cell biology Cell signaling Eye Diseases Ocular Anatomy lcsh:Medicine Surgical and Invasive Medical Procedures Signal transduction Epithelium Cornea Aqueous Humor Transforming Growth Factor beta2 Ocular System Animal Cells Medicine and Health Sciences Confidence Intervals Humans Endothelium lcsh:Science Corneal Dystrophies Hereditary Biology and life sciences Endothelium Corneal lcsh:R Ophthalmic Procedures Signaling cascades Endothelial Cells Glaucoma Epithelial Cells Cataract Surgery Middle Aged Ophthalmology Biological Tissue TGF-beta signaling cascade Physical Sciences Cardiovascular Anatomy Eyes Female lcsh:Q Anatomy Cellular Types Head Mathematics Statistics (Mathematics) Keratoplasty Penetrating Research Article |
| Zdroj: | PLoS ONE, Vol 12, Iss 4, p e0175509 (2017) PLoS ONE |
| ISSN: | 1932-6203 |
| Popis: | Purpose Posterior polymorphous corneal dystrophy (PPCD) is characterized by abnormal proliferation of corneal endothelial cells. It was shown that TGF-β2 present in aqueous humor (AH) could help maintaining the corneal endothelium in a G1-phase-arrest state. We wanted to determine whether the levels of this protein are changed in AH of PPCD patients. Methods We determined the concentrations of active TGF-β2 in the AH of 29 PPCD patients (42 samples) and 40 cadaver controls (44 samples) by ELISA. For data analysis the PPCD patients were divided based on either the molecular genetic cause of their disease as PPCD1 (37 samples), PPCD3 (1 sample) and PPCDx (not linked to a known PPCD loci, 4 samples) or on the presence (17 samples) or absence (25 samples) of secondary glaucoma or on whether they had undergone penetrating keratoplasty (PK, 32 samples) or repeated PK (rePK, 7 samples). Results The level of active TGF-β2 in the AH of all PPCD patients (mean ± SD; 386.98 ± 114.88 pg/ml) in comparison to the control group (260.95 ± 112.43 pg/ml) was significantly higher (P = 0.0001). Compared to the control group, a significantly higher level of active TGF-β2 was found in the PPCD1 (P = 0.0005) and PPCDx (P = 0.0022) groups. Among patients the levels of active TGF-β2 were not significantly affected by gender, age, secondary glaucoma or by the progression of dystrophy when one or repeated PK were performed. Conclusion The levels of active TGF-β2 in the AH of PPCD patients are significantly higher than control values, and thus the increased levels of TGF-β2 could be a consequence of the PPCD phenotype and can be considered as another feature characterizing this disease. |
| Databáze: | OpenAIRE |
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