| Autor: |
A A, Terent'ev, G V, Kostyuk, P Y, Boikov |
| Předmět: |
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| Zdroj: |
Publons |
| Popis: |
Dynamics of structural changes in the chromatin of rat liver cells were studied during activation of the c-myc, c-fos, and c-jun protooncogenes. Nuclei were isolated at various stages of gene activation using cycloheximide and chromatin was subjected to partial nucleolysis by endogenous Ca2+/Mg2+-DNAse and extracted with low magnesium buffer; the nucleosome fragmentation was analyzed. DNA was isolated from the soluble chromatin and low-molecular-weight fragments were separated by electrophoresis through a composite gel system (3 and 1% agarose). This modification of DNA electrophoresis protocol improves the efficiency of separation of the low-molecular-weight DNA fragments. Increased protooncogene activity is associated with decreased content of DNAse-sensitive soluble chromatin. The fractional content of the c-fos gene is increased in the soluble chromatin. In control nuclei, endogenous Ca2+/Mg2+-DNAse cleaves mononucleosomes containing DNA fragments with variable length. Upon protooncogene activation, mono- and oligonucleosomes with elongated DNA fragments are predominantly formed. The changes involve about 40% of the nuclear chromatin. The data suggest that protooncogene activation is associated with reorganization at the nucleosome level of the structural organization of the chromatin. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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