Prodrug-activated gene therapy: involvement of an immunological component in the 'bystander effect'
| Autor: | Gagandeep S, Brew R, Green B, Steve Christmas, Klatzmann D, Gj, Poston, Ar, Kinsella |
|---|---|
| Rok vydání: | 1996 |
| Předmět: |
Mice
Inbred BALB C Genetic Vectors CD4-CD8 Ratio Down-Regulation Mice Nude Genetic Therapy Adenocarcinoma Immunohistochemistry Thymidine Kinase Statistics Nonparametric Mice Lymphocytes Tumor-Infiltrating Phenotype Colonic Neoplasms Immune Tolerance Tumor Cells Cultured Animals Simplexvirus Female Prodrugs Ganciclovir |
| Zdroj: | Europe PubMed Central |
| ISSN: | 0929-1903 |
| Popis: | The integration and expression of the herpes simplex virus type 1 thymidine kinase (HSV1-TK) gene in localized tumors results in tumor regression after the administration of the specific nucleoside analogue ganciclovir (GCV). Although only 10% to 20% of the tumor cells take up the HSV1-TK gene, the neighboring cells also die, a phenomenon termed "bystander effect.". In the present study, coinjection of the MC26 mouse colon carcinoma cell line and the HSV1-TK expressing retroviral packaging cell line followed after 7 days by the intraperitoneal administration of GCV resulted in almost total tumor regression in the immunocompetent BALB/c mice but not in immunocompromised athymic BALB/c mice. This suggested a strong cell-mediated immune component to the bystander effect. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|