[Creation of a heterologous gene expression system on the basis of Aspergillus awamori recombinant strain]
| Autor: | Am, Rozhkova, As, Sereda, Nv, Tsurikova, Ak, Nurtaeva, Mv, Semenova, Lv, Rimareva, Ea, Rubtsova, Ivan Zorov, Oa, Sinitsyna, Ap, Sinitsyn |
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| Předmět: |
Terminator Regions
Genetic Trichoderma 6-Phytase Base Sequence Genetic Vectors Molecular Sequence Data Penicillium Recombinant Proteins Xylan Endo-1 3-beta-Xylosidase Aspergillus Cellulase Gene Expression Regulation Fungal Cloning Molecular Glucan 1 4-alpha-Glucosidase Genetic Engineering Promoter Regions Genetic Biotechnology |
| Zdroj: | Europe PubMed Central |
| Popis: | A heterologous gene expression system was created in a domestic Aspergillus awamori Co-6804 strain, which is a producer of the glucoamylase gene. Vector pGa was prepared using promoter and terminator areas of the glucoamylase gene, and A. niger phytase, Trichoderma reesei endoglucanase, and Penicillium canescens xylanase genes were then cloned into pGa vector. Separation of enzyme samples using FPLC showed the amount of the recombinant proteins to be within the 0.6-14% range of total protein. |
| Databáze: | OpenAIRE |
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