GTSE1 Is a Microtubule Plus-End Tracking Protein That Regulates EB1-Dependent Cell Migration
| Autor: | Scolz, Massimilano, Widlund, Per O., Piazza, Silvano, Bublik, Debora Rosa, Reber, Simone, Peche, Leticia Y., Ciani, Yari, Hubner, Nina, Isokane, Mayumi, Monte, Martin, Ellenberg, Jan, Hyman, Anthony A., Schneider, Claudio, Bird, Alexander W. |
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| Jazyk: | angličtina |
| Rok vydání: | 2012 |
| Předmět: |
cell migration
lcsh:Medicine Fluorescent Antibody Technique Kaplan-Meier Estimate cancer cell culture MIGRACION Biochemistry Microtubules Mass Spectrometry Metastasis purl.org/becyt/ford/1 [https] Cell Movement Molecular Cell Biology Basic Cancer Research Morphogenesis focal adhesion RNA Small Interfering lcsh:Science article Obstetrics and Gynecology protein function microtubule plus end tracking protein Extracellular Matrix unclassified drug Gene Expression Regulation Neoplastic Cell Motility Oncology protein protein interaction Medicine Female RNA Interference Microtubule-Associated Proteins CIENCIAS NATURALES Y EXACTAS Research Article microtubule regulatory mechanism Otras Ciencias Biológicas Biophysics binding protein Breast Neoplasms Cell Migration Real-Time Polymerase Chain Reaction Cell Line Ciencias Biológicas breast cancer Breast Cancer Cell Adhesion Humans Immunoprecipitation controlled study Neoplasm Invasiveness human purl.org/becyt/ford/1.6 [https] Biology Extracellular Matrix Adhesions DNA Primers microtubule protein human cell Gene Expression Profiling lcsh:R Proteins nucleotide sequence Regulatory Proteins Cytoskeletal Proteins G2 and S phase expressed 1 protein Microscopy Fluorescence METASTASIS lcsh:Q end binding 1 protein GTSE MICROTUBULOS microtubule associated protein Developmental Biology |
| Zdroj: | PLoS ONE 2012;7(12) Biblioteca Digital (UBA-FCEN) Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturales instacron:UBA-FCEN CONICET Digital (CONICET) Consejo Nacional de Investigaciones Científicas y Técnicas instacron:CONICET PLoS ONE, Vol 7, Iss 12, p e51259 (2012) PLoS ONE |
| Popis: | The regulation of cell migration is a highly complex process that is often compromised when cancer cells become metastatic. The microtubule cytoskeleton is necessary for cell migration, but how microtubules and microtubule-associated proteins regulate multiple pathways promoting cell migration remains unclear. Microtubule plus-end binding proteins (+TIPs) are emerging as important players in many cellular functions, including cell migration. Here we identify a +TIP, GTSE1, that promotes cell migration. GTSE1 accumulates at growing microtubule plus ends through interaction with the EB1+TIP. The EB1-dependent +TIP activity of GTSE1 is required for cell migration, as well as for microtubule-dependent disassembly of focal adhesions. GTSE1 protein levels determine the migratory capacity of both nontransformed and breast cancer cell lines. In breast cancers, increased GTSE1 expression correlates with invasive potential, tumor stage, and time to distant metastasis, suggesting that misregulation of GTSE1 expression could be associated with increased invasive potential. © 2012 Scolz et al. Fil: Scolz, Massimilano. Laboratorio Nazionale The Interuniversity Consortium for Biotechnology; Italia Fil: Widlund, Per O.. Max Planck Institute of Molecular Cell Biology and Genetics; Alemania Fil: Piazza, Silvano. Laboratorio Nazionale The Interuniversity Consortium for Biotechnology; Italia Fil: Bublik, Debora Rosa. Laboratorio Nazionale The Interuniversity Consortium for Biotechnology; Italia Fil: Reber, Simone. Max Planck Institute of Molecular Cell Biology and Genetics; Alemania Fil: Peche, Leticia Y.. Laboratorio Nazionale The Interuniversity Consortium for Biotechnology; Italia Fil: Ciani, Yari. Laboratorio Nazionale The Interuniversity Consortium for Biotechnology; Italia Fil: Hubner, Nina. Universitair Medisch Centrum Utrecht; Países Bajos Fil: Isokane, Mayumi. European Molecular Biology Laboratory; Alemania Fil: Monte, Martin. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Laboratorio Nazionale The Interuniversity Consortium for Biotechnology; Italia Fil: Ellenberg, Jan. European Molecular Biology Laboratory; Alemania Fil: Hyman, Anthony A.. Max Planck Institute of Molecular Cell Biology and Genetics; Alemania Fil: Schneider, Claudio. Laboratorio Nazionale The Interuniversity Consortium for Biotechnology; Italia. University of Udine; Italia Fil: Bird, Alexander W.. Max Planck Institute of Molecular Cell Biology and Genetics; Alemania |
| Databáze: | OpenAIRE |
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