Correlation between invasiveness of colorectal tumor cells and adhesive potential under flow
| Autor: | Benoliel, A. M., Pirro, N., Marin, V., Consentino, B., Pierre Bongrand, Vitte, J., Bongrand, P., Sielezneff, I., Sastre, B. |
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| Přispěvatelé: | Adhésion et Inflammation (LAI), Assistance Publique - Hôpitaux de Marseille (APHM)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Aix Marseille Université (AMU)-Centre National de la Recherche Scientifique (CNRS), Service de Chirurgie Digestive (AP-HM), Université de la Méditerranée - Aix-Marseille 2, Bongrand, Pierre, Aix Marseille Université (AMU)-Assistance Publique - Hôpitaux de Marseille (APHM)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS) |
| Jazyk: | angličtina |
| Rok vydání: | 2003 |
| Předmět: |
MESH: Antigens
CD29 MESH: E-Selectin Vascular Cell Adhesion Molecule-1 [SDV.BBM.BP] Life Sciences [q-bio]/Biochemistry Molecular Biology/Biophysics MESH: Flow Cytometry [SDV.CAN]Life Sciences [q-bio]/Cancer MESH: Cadherins MESH: Cell Adhesion [SDV.CAN] Life Sciences [q-bio]/Cancer Antigens CD Cell Adhesion Humans Neoplasm Invasiveness MESH: Antigens CD MESH: Antigens CD18 MESH: Colonic Neoplasms MESH: Humans MESH: Stress Mechanical Rectal Neoplasms Integrin beta1 MESH: Vascular Cell Adhesion Molecule-1 MESH: Biological Markers MESH: Rectal Neoplasms MESH: Neoplasm Invasiveness Cadherins Flow Cytometry [SDV.BBM.BP]Life Sciences [q-bio]/Biochemistry Molecular Biology/Biophysics CD18 Antigens Colonic Neoplasms MESH: Cell Adhesion Molecules Stress Mechanical E-Selectin Cell Adhesion Molecules Biomarkers |
| Zdroj: | Anticancer Research Anticancer Research, International Institute of Anticancer Research, 2003, 23 (6C), pp.4891-6 Anticancer Research, 2003, 23 (6C), pp.4891-6 ResearcherID |
| ISSN: | 0250-7005 |
| Popis: | International audience; BACKGROUND: Tumor cell adhesiveness is involved in metastatic dissemination, and adhesive behavior may be different under static and dynamic conditions. MATERIALS AND METHODS: Patients undergoing primary colorectal cancer excision were tested for: i) serum concentration of sE-selectin, sICAM-1 and sVCAM-1, ii) expression of CD18, CD29d and E-cadherin on tumor cells and iii) efficiency of tumor cell adhesion to ECV304 monolayers under flow and resistance to detachment by shear. RESULTS: Twenty out of 31 patients were free of detectable relapse 12 months later. Relapsing and non-relapsing patients had similar levels of soluble adhesion molecules. E-cadherin was detected on tumor cells from three non-relapsing patients, but no relapsing one. Unexpectedly, significant CD18 labeling was found on two relapsing patients and one non-relapsing patient. Cells from relapsing patients displayed significantly increased (p < 0.05 two-sided, p < 0.025 one-sided) capacity to adhere to test monolayers under flow. CONCLUSION: Cancer invasion is related to tumor cell adhesiveness, and the flow chamber provides a practical way of measuring adhesive parameters with a potential value for relapse prediction. |
| Databáze: | OpenAIRE |
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