Titanium nitride oxide coating on rough titanium stimulates the proliferation of human primary osteoblasts

Autor: Durual, Stéphane, Pernet, F., Rieder, Philippe, Mekki, Mustapha, Cattani-Lorente, Maria Angeles, Wiskott, Anselm
Rok vydání: 2010
Předmět:
Alloys/chemistry
Coated Materials
Biocompatible/chemistry

Plasma Gases
Dental Etching/methods
Surface Properties
Osteocalcin
Cell Count
Hydrochloric Acid/chemistry
Plasma Gases/chemistry
Cell Proliferation/drug effects
Dental Materials
Acid Etching
Dental

Calcitriol
Coated Materials
Biocompatible

Alloys
Aluminum Oxide
Cell Adhesion
Humans
Calcitriol/pharmacology
Cell Adhesion/physiology
Cells
Cultured

Cell Proliferation
Titanium
Osteoblasts
Osteoprotegerin/analysis
Reverse Transcriptase Polymerase Chain Reaction
Photoelectron Spectroscopy
technology
industry
and agriculture

Osteoprotegerin
Alkaline Phosphatase/analysis
Cell Differentiation
respiratory system
equipment and supplies
Alkaline Phosphatase
ddc:617.6
Osteoblasts/drug effects/physiology
Aluminum Oxide/chemistry
Osteocalcin/analysis
Titanium/chemistry
Calcium Channel Agonists
Gene Expression Regulation
Dental Materials/chemistry
Dental Etching
Microscopy
Electron
Scanning

Cell Differentiation/drug effects/physiology
Hydrochloric Acid
Acid Etching
Dental/methods

Calcium Channel Agonists/pharmacology
Zdroj: Clinical Oral Implants Research, Vol. 22, No 5 (2011) pp. 552-9
ISSN: 1600-0501
0905-7161
Popis: Titanium is widely used in contemporary endosseous implantology and there is considerable thrust to further promote osseointegration by implant surface modifications. The aim of this study was to evaluate the effect of a titanium-nitride-oxide (TiNOx) coating on commercially pure microroughened titanium by assessing the proliferation and differentiation of human primary osteoblasts.Cell proliferation, gene expression, alkaline phosphatase activity, osteoprotegerin and osteocalcin secretion were analyzed for a time course of 3 weeks, with or without additional stimulation by 1.25(OH)(2) vitamin D(3) 100 nM.A 1.5-fold increase in the proliferation rate of cells grown on TiNOx-coated titanium as compared with uncoated surfaces was observed. SEM views indicated that the cells' normal morphology with their numerous extensions was maintained. The differentiation process on the TiNOx surface was only affected to a minor degree and translated into a slight delay in osteoblast maturation when compared to uncoated titanium.Pending confirmation of these results in vivo, TiNOx coatings could potentially accelerate and enhance osseointegration.
Databáze: OpenAIRE