Titanium nitride oxide coating on rough titanium stimulates the proliferation of human primary osteoblasts
Autor: | Durual, Stéphane, Pernet, F., Rieder, Philippe, Mekki, Mustapha, Cattani-Lorente, Maria Angeles, Wiskott, Anselm |
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Rok vydání: | 2010 |
Předmět: |
Alloys/chemistry
Coated Materials Biocompatible/chemistry Plasma Gases Dental Etching/methods Surface Properties Osteocalcin Cell Count Hydrochloric Acid/chemistry Plasma Gases/chemistry Cell Proliferation/drug effects Dental Materials Acid Etching Dental Calcitriol Coated Materials Biocompatible Alloys Aluminum Oxide Cell Adhesion Humans Calcitriol/pharmacology Cell Adhesion/physiology Cells Cultured Cell Proliferation Titanium Osteoblasts Osteoprotegerin/analysis Reverse Transcriptase Polymerase Chain Reaction Photoelectron Spectroscopy technology industry and agriculture Osteoprotegerin Alkaline Phosphatase/analysis Cell Differentiation respiratory system equipment and supplies Alkaline Phosphatase ddc:617.6 Osteoblasts/drug effects/physiology Aluminum Oxide/chemistry Osteocalcin/analysis Titanium/chemistry Calcium Channel Agonists Gene Expression Regulation Dental Materials/chemistry Dental Etching Microscopy Electron Scanning Cell Differentiation/drug effects/physiology Hydrochloric Acid Acid Etching Dental/methods Calcium Channel Agonists/pharmacology |
Zdroj: | Clinical Oral Implants Research, Vol. 22, No 5 (2011) pp. 552-9 |
ISSN: | 1600-0501 0905-7161 |
Popis: | Titanium is widely used in contemporary endosseous implantology and there is considerable thrust to further promote osseointegration by implant surface modifications. The aim of this study was to evaluate the effect of a titanium-nitride-oxide (TiNOx) coating on commercially pure microroughened titanium by assessing the proliferation and differentiation of human primary osteoblasts.Cell proliferation, gene expression, alkaline phosphatase activity, osteoprotegerin and osteocalcin secretion were analyzed for a time course of 3 weeks, with or without additional stimulation by 1.25(OH)(2) vitamin D(3) 100 nM.A 1.5-fold increase in the proliferation rate of cells grown on TiNOx-coated titanium as compared with uncoated surfaces was observed. SEM views indicated that the cells' normal morphology with their numerous extensions was maintained. The differentiation process on the TiNOx surface was only affected to a minor degree and translated into a slight delay in osteoblast maturation when compared to uncoated titanium.Pending confirmation of these results in vivo, TiNOx coatings could potentially accelerate and enhance osseointegration. |
Databáze: | OpenAIRE |
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