Differentiation of Bovine Spermatogonial Stem Cells into Osteoblasts

Autor:	Qasemi-Panahi, B., Tajik, P., Movahedin, M., Moghaddam, G., Barzgar, Y., Hamed Heidari-Vala
Jazyk:	angličtina
Rok vydání:	2011
Předmět:	endocrine system Differentiation Osteoblast Original Article Spermatogonial stem cell
Zdroj:	Avicenna Journal of Medical Biotechnology Scopus-Elsevier
ISSN:	2008-4625 2008-2835
Popis:	Spermatogonial Stem Cell (SSC) technologies provide multiple opportunities for research in the field of biotechnology and regenerative medicine. The therapeutic use of Embryonic Stem Cells (ESCs) is restricted due to severe ethical and immunological concerns. Therefore, we need a new pluripotent cell type. Despite well-known role of germ cells in the gametogenesis, some facts apparently show their multipotentiality. In the present study, bovine SSCs were co-cultured with Sertoli cell for 7 days. Sertoli cells and SSCs were identified by Vimentin and Oct-4 immunocytochemical staining method, respectively. In order to differentiate SSCs into osteoblasts, we used consecutive inducer media without separation of the colonies. We characterized osteoblasts using Alizarin red staining.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=pmid_dedup__::1ca24b8aab0a33c2d85d79085f179dd7 http://europepmc.org/articles/PMC3558187 Zobrazit plný text záznamu Plný text ve formátu PDF