| Autor: |
Cobellis, G., Vallarino, Mauro, Meccariello, R., Pierantoni, R., Masini, MARIA ANGELA, Mathieu, M., PERNAS ALONSO, R., Chieffi, P., Fasano, S. |
| Přispěvatelé: |
Cobellis, Gilda, Vallarino, M, Meccariello, R, Pierantoni, Riccardo, Masini, Ma, Mathieu, M, PERNAS ALONSO, R, Chieffi, Paolo, Fasano, Silvia |
| Rok vydání: |
1999 |
| Předmět: |
|
| Zdroj: |
Journal of neuroendocrinology. 11(9) |
| ISSN: |
0953-8194 |
| Popis: |
C-fos activity was determined in the brain of the frog, Rana esculenta, during the annual sexual cycle. The localization of GnRH molecular forms (mammalian- and chicken-GnRHII) was also carried out to determine whether or not the proto-oncogene and the peptides showed a functional relationship. Northern blot analysis of total RNA revealed the presence of a single strong signal of c-fos like mRNA of 1.9 Kb during February and April. This was followed by expression of c-Fos protein (Fos) in several brain areas during March and July shown by immunocytochemistry. In particular, the olfactory region, the lateral and medial pallium, the nucleus lateralis septi, the ventral striatum, the caudal region of the anterior preoptic area, the suprachiasmatic nucleus, the ventral thalamus, tori semicircularis and ependymal layers of the tectum were immunostained. There was no overlap between Fos immunoreactive perikarya and GnRH immunoreactive perikarya (e.g. gonadotrophin-releasing hormone (GnRH) in the rostral part and Fos in the caudal region of the anterior preoptic area). Interestingly, a cytoplasmic localization of Fos was also observed by immunocytochemistry and gel retardation experiments supported this observation. Cytoplasmic extracts from September-October animals bound the AP1 oligonucleotide. The complex was not available in the nuclear extracts from the same preparation, suggesting that, besides Fos, Jun products were also present. Conversely, nuclear but not cytosolic binding was detected in the brain of animals collected in July. In conclusion, we show that Fos and GnRH activity does not correlate in the frog brain and, for the first time in a vertebrate species, we give evidence of a cytoplasmic AP1 complex in neuronal cells. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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