[Biosynthesis of mini-antibodies to human ferritin in plant and bacterial producers]

Autor:	Eg, Semeniuk, Oa, Stremovskiĭ, Iv, Orlova, Tg, Baladin, Am, Nosov, Bur'ianov IaI, Sergey Deyev, Rv, Petrov
Rok vydání:	2003
Předmět:	Ribonucleases Bacteria Bacterial Proteins Base Sequence Recombinant Fusion Proteins Ferritins Humans Proteins Plants Immunoglobulin Fragments Chromatography Affinity DNA Primers
Zdroj:	Europe PubMed Central
ISSN:	0026-8984
Popis:	A recombinant scFv antibody against human spleen ferritin was expressed as a barstar-fused protein in Escherichia coli and in Nicotiana tabacum plants and suspension cell cultures. As demonstrated by immunoblotting with antibarstar antibodies, direction of the recombinant protein to the endomembrane system of plant cells ensured its stability and solubility. Production of the recombinant protein did not differ between parental transgenic plants and their first-generation progeny. Fusion with barstar allowed not only immunochemical detection of the recombinant scFv antibody, but also their purification from the plant material by affinity chromatography with barnase-His6 immobilized on a metal-affinity carrier.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=pmid_dedup__::1ad87ff027394c862551f768dda52139 https://pubmed.ncbi.nlm.nih.gov/14593930 Zobrazit plný text záznamu