Real-time monitoring of bacterial growth kinetics in suspensions using laser speckle imaging

Autor: Loutfi, Hadi, Pellen, Fabrice, Le Jeune, Bernard, Lteif, Roger, Kallassy, Mireille, Le Brun, Guy, Abboud, Marie
Přispěvatelé: Laboratoire d'Optique et de Magnétisme (OPTIMAG), Université de Brest (UBO)-Centre Hospitalier Régional Universitaire de Brest (CHRU Brest)-Institut Brestois Santé Agro Matière (IBSAM), Université de Brest (UBO), Université Saint-Joseph de Beyrouth (USJ)
Jazyk: angličtina
Rok vydání: 2020
Předmět:
Zdroj: Scientific Reports, Vol 10, Iss 1, Pp 1-10 (2020)
Scientific Reports
Scientific Reports, Nature Publishing Group, 2020, 10 (1), ⟨10.1038/s41598-019-57281-2⟩
ISSN: 2045-2322
Popis: International audience; In microbiology, monitoring the growth of any microorganism in culture is important for studying and optimizing the growth kinetics, the biomass and the metabolite production. In this work, we show that laser speckle imaging is a reliable technique that can be used to perform real-time monitoring of bacteria growth kinetic in liquid culture media. Speckle parameters, specifically speckle grain size and the spatial contrast of the speckle images, and standard analytical parameters (optical density, pH and colony forming units) were measured during the culture of different strains of Bacillus thuringiensis . Our results show that both speckle grain size and spatial contrast decrease with bacterial growth. Furthermore, speckle parameters are sensitive to the fermentation conditions. Statistical analysis revealed a relatively high correlation between speckle and analytical parameters.
Databáze: OpenAIRE
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