Heterochromatic marks are associated with the repression of secondary metabolism clusters in Aspergillus nidulans
| Autor: | Reyes-Dominguez, Yazmid, Bok, Jin Woo, Berger, Harald, Shwab, E Keats, Basheer, Asjad, Gallmetzer, Andreas, Scazzocchio, Claudio, Keller, Nancy, Strauss, Joseph |
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| Přispěvatelé: | Institut de génétique et microbiologie [Orsay] (IGM), Université Paris-Sud - Paris 11 (UP11)-Centre National de la Recherche Scientifique (CNRS) |
| Jazyk: | angličtina |
| Rok vydání: | 2010 |
| Předmět: |
MESH: Histones
MESH: Indoles Indoles MESH: Aspergillus nidulans Sterigmatocystin Acetylation Penicillins Methylation Aspergillus nidulans MESH: Methylation Fungal Proteins Histones MESH: Heterochromatin [SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology MESH: Penicillins Gene Expression Regulation Fungal Heterochromatin Multigene Family MESH: Sterigmatocystin MESH: Multigene Family MESH: Fungal Proteins [SDV.BBM]Life Sciences [q-bio]/Biochemistry Molecular Biology MESH: Acetylation MESH: Gene Expression Regulation Fungal Research Articles |
| Zdroj: | Molecular Microbiology Molecular Microbiology, Wiley, 2010, 76 (6), pp.1376-86. ⟨10.1111/j.1365-2958.2010.07051.x⟩ |
| ISSN: | 1365-2958 0950-382X |
| DOI: | 10.1111/j.1365-2958.2010.07051.x⟩ |
| Popis: | International audience; Fungal secondary metabolites are important bioactive compounds but the conditions leading to expression of most of the putative secondary metabolism (SM) genes predicted by fungal genomics are unknown. Here we describe a novel mechanism involved in SM-gene regulation based on the finding that, in Aspergillus nidulans, mutants lacking components involved in heterochromatin formation show de-repression of genes involved in biosynthesis of sterigmatocystin (ST), penicillin and terrequinone A. During the active growth phase, the silent ST gene cluster is marked by histone H3 lysine 9 trimethylation and contains high levels of the heterochromatin protein-1 (HepA). Upon growth arrest and activation of SM, HepA and trimethylated H3K9 levels decrease concomitantly with increasing levels of acetylated histone H3. SM-specific chromatin modifications are restricted to genes located inside the ST cluster, and constitutive heterochromatic marks persist at loci immediately outside the cluster. LaeA, a global activator of SM clusters in fungi, counteracts the establishment of heterochromatic marks. Thus, one level of regulation of the A. nidulans ST cluster employs epigenetic control by H3K9 methylation and HepA binding to establish a repressive chromatin structure and LaeA is involved in reversal of this heterochromatic signature inside the cluster, but not in that of flanking genes. |
| Databáze: | OpenAIRE |
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