Bv8, the amphibian homologue of the human prokineticins, induces a proinflammatory phenotype of mouse macrophages
| Autor: | Martucci, Cataldo, Franchi, Silvia, Giannini, Elisa, Tian, Hui, Melchiorri, Pietro, Negri, Lucia, Sacerdote, Paola |
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| Jazyk: | angličtina |
| Rok vydání: | 2006 |
| Předmět: |
Lipopolysaccharides
Male Mice Knockout prokineticins Mice Inbred BALB C Bv8 chemotaxis anti-inflammatory cytokines macrophages proinflammatory cytokines Tumor Necrosis Factor-alpha Interleukin-1beta Neuropeptides In Vitro Techniques Interleukin-12 Amphibian Proteins Receptors G-Protein-Coupled Gastrointestinal Hormones Mice Papers Macrophages Peritoneal Animals Cytokines |
| Popis: | 1.--The small protein Bv8, isolated from the amphibian skin, belongs to a novel family of secreted proteins linked to several biological effects. We describe the expression of Bv8/prokineticins and their receptors in mouse macrophages, and characterize their proinflammatory activities. 2.--The rodent analogue of Bv8, prokineticin-2, is expressed by macrophages, as well as its G-protein-coupled receptor prokineticin receptor (PKR-1 and PKR-2). PKR-1 is expressed more abundantly. 3.-- Bv8 induces potent chemotaxis of macrophages at concentrations as low as 10(-12) M. 4.-- It stimulates lipopolysaccharide-induced production of the proinflammatory cytokines IL-1 and IL-12, reducing that of the anti-inflammatory cytokine IL-10. The effects are observed starting at the very low concentration of 10(-11) M. 5.--Effects on chemotaxis and cytokine are not pertussis-toxin sensitive, but are completely prevented by addition of the phospholipase inhibitor U73122, suggesting a G(q) protein is involved in the Bv8-induced effects. 6.--Studies in PKR-1 knockout mice indicate that all the activities exerted by Bv8 on macrophages are mediated by the PKR-1 receptor. 7.--In conclusion, Bv8 appears to be able to induce the macrophage to migrate and to acquire a proinflammatory phenotype. |
| Databáze: | OpenAIRE |
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