Analyzing protein distribution in plant tissues using 'whole-mount' immunolocalization
| Autor: | Pilar, Bustos-Sanmamed, Carole, Laffont, Florian, Frugier, Christine, Lelandais-Brière, Martin, Crespi |
|---|---|
| Přispěvatelé: | Institut des sciences du végétal ( ISV ), Centre National de la Recherche Scientifique ( CNRS ), Institut des sciences du végétal (ISV), Centre National de la Recherche Scientifique (CNRS) |
| Jazyk: | angličtina |
| Rok vydání: | 2013 |
| Předmět: |
[ SDV.BV ] Life Sciences [q-bio]/Vegetal Biology
RNA Untranslated animal structures MESH : RNA Untranslated MESH : Ribonucleoproteins Small Nuclear MESH: Plant Proteins MESH: Ribonucleoproteins Small Nuclear MESH : Medicago truncatula Ribonucleoproteins Small Nuclear MESH: RNA Untranslated MESH: Ribonucleoproteins Ribonucleoproteins MESH : Spliceosomes MESH : Plant Proteins Medicago truncatula Spliceosomes [SDV.BV]Life Sciences [q-bio]/Vegetal Biology MESH : Ribonucleoproteins MESH: Medicago truncatula MESH: Spliceosomes Plant Proteins |
| Zdroj: | Methods in Molecular Biology (Clifton then Totova) Methods in Molecular Biology (Clifton then Totova), Humana Press (Springer Imprint), 2013, 959, pp.317-22. 〈10.1007/978-1-62703-221-6_21〉 Methods in Molecular Biology Methods in Molecular Biology, Humana Press/Springer Imprint, 2013, 959, pp.317-22. ⟨10.1007/978-1-62703-221-6_21⟩ |
| ISSN: | 1064-3745 1940-6029 |
| DOI: | 10.1007/978-1-62703-221-6_21〉 |
| Popis: | International audience; Proteins are distributed in different cellular compartments. Our group studies the role of non-coding RNAs and associated RNPs in the development and stress response in legumes. Ribonucleoproteins (RNPs) are RNA-protein complexes that play different roles in many cellular processes. Long and small non-coding RNAs determine the specificity of action of several RNPs as the RNA Induced Silencing Complex (RISC), or affect mRNA translation, splicing and stability by interacting with other RNPs such as P-bodies, spliceosome or polysomes. Together with small and long RNAs (Chapter 20), the precise localization of the associated RNPs or the translational products regulated by small RNAs (ie target proteins regulated by miRNAs, or translationally-regulated products) by immunocytochemistry could bring novel insights into these regulatory processes. The protocol described is currently used for detection of RNP associated proteins in nodules and roots of Medicago truncatula but could be extended to any other protein. The critical points, as the choice of the antibody and the fixation and permeabilization steps, that allow preservation of tissue and cell integrity and increase the accessibility to epitopes, will be discussed. |
| Databáze: | OpenAIRE |
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