Hypoxia-regulated microRNAs in gastroesophageal cancer
| Autor: | Mette Winther, Jan Alsner, Brita Singers Sørensen, Catja Foged Wittrup, Trine Tramm, Beaksgaard, L., Hofland, K., Holtved, E., Marianne Nordsmark |
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| Předmět: |
Adult
Aged 80 and over Male Esophageal Neoplasms Gene Expression Profiling Kaplan-Meier Estimate Adenocarcinoma Middle Aged Real-Time Polymerase Chain Reaction Cell Hypoxia Gene Expression Regulation Neoplastic MicroRNAs Treatment Outcome Cell Line Tumor Carcinoma Squamous Cell Humans Female Esophageal Squamous Cell Carcinoma Aged Oligonucleotide Array Sequence Analysis Proportional Hazards Models Retrospective Studies |
| Zdroj: | Aarhus University Scopus-Elsevier Winther, M, Alsner, J, Sørensen, B S, Wittrup, C F, Tramm, T, Beaksgaard, L, Hofland, K, Holtved, E & Nordsmark, M 2016, ' Hypoxia-regulated microRNAs in gastroesophageal cancer ', Anticancer Research, vol. 36, no. 2, pp. 721-30 . < http://ar.iiarjournals.org/content/36/2/721 > Winther, M, Alsner, J, Sørensen, B S, Wittrup, C F, Tramm, T, Bæksgaard, L, Hofland, K, Holtved, E & Nordsmark, M 2016, ' Hypoxia-regulated MicroRNAs in gastroesophageal cancer ', Anticancer Research, vol. 36, no. 2, pp. 721-730 . < http://ar.iiarjournals.org/content/36/2/721.abstract?sid=fdaddca0-9804-402a-aecd-c950272ff83e > |
| Popis: | The present study aimed to identify hypoxia-regulated microRNAs (HRMs) in vitro and investigate the clinical role of candidate HRMs in patients with gastroesophageal cancer (GEC).microRNA expression changes induced by hypoxia in human GEC cell lines were measured with microarrays and validated by quantitative real-time polymerase chain reaction. Candidate HRMs were measured in pre-therapeutic tumor samples from 195 patients with GEC.Expression of miR-210 was shown to be significantly induced in esophageal squamous cell carcinoma (9.26-fold, p0.001) and adenocarcinoma cell lines (4.95-fold, p0.001) and miR-27a-star was significantly up-regulated in adenocarcinoma cell lines (4.79-fold, p=0.04). A weak but significant correlation between miR-210 expression and a 15-gene hypoxia signature was observed (Pearson r correlation: r=0.38, p0.001). No significant associations of HRMs and clinical outcome in patients with GEC were identified.This study supports the involvement of hypoxia on miRNAs in vitro and confirms the role of miR-210 as being a universal HRM. |
| Databáze: | OpenAIRE |
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