Evidence for the negative regulation of phytase gene expression in Streptomyces lividans and Streptomyces coelicolor

Autor: Boukhris, Ines, Dulermo, Thierry, Chouayekh, Hichem, Virolle, Marie-Joëlle
Přispěvatelé: Centre de Biotechnologie de Sfax ( CBS ), Institut de Biologie Intégrative de la Cellule ( I2BC ), Université Paris-Saclay-Centre National de la Recherche Scientifique ( CNRS ) -Commissariat à l'énergie atomique et aux énergies alternatives ( CEA ) -Université Paris-Sud - Paris 11 ( UP11 ), Centre de Biotechnologie de Sfax (CBS), Institut de Biologie Intégrative de la Cellule (I2BC), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS)
Jazyk: angličtina
Rok vydání: 2016
Předmět:
MESH : Operon
[SDV]Life Sciences [q-bio]
MESH: Base Sequence
MESH : Promoter Regions
Genetic
MESH : Glucuronidase
MESH: Glucuronidase
MESH : Bacterial Proteins
MESH: 6-Phytase
MESH : Streptomyces coelicolor
Promoter Regions
Genetic
MESH: Bacterial Proteins
Soil Microbiology
Glucuronidase
Sequence Deletion
6-Phytase
MESH: Gene Expression Regulation
Bacterial
MESH : Protein Binding
MESH: Sequence Deletion
Negative
Protein Binding
MESH : Gene Expression Regulation
Bacterial
MESH: Operon
Phytic Acid
MESH : Soil Microbiology
MESH: Streptomyces lividans
Streptomyces coelicolor
Beta-glucuronidase reporter gene
Bacterial Proteins
PHO box
PhoR
PhoP
Operon
MESH: Promoter Regions
Genetic
MESH: Protein Binding
MESH: Phytic Acid
Repetitive Sequences
Nucleic Acid
MESH : Streptomyces lividans
MESH: Repetitive Sequences
Nucleic Acid
Base Sequence
[ SDV ] Life Sciences [q-bio]
MESH : 6-Phytase
MESH : Sequence Deletion
Gene Expression Regulation
Bacterial
MESH: Streptomyces coelicolor
MESH : Repetitive Sequences
Nucleic Acid
MESH : Phytic Acid
MESH: Soil Microbiology
Streptomyces lividans
MESH : Base Sequence
Streptomyces phytase
Positive regulation
Phosphate limitation
Zdroj: Journal of Basic Microbiology
Journal of Basic Microbiology, Wiley-VCH Verlag, 2016, 56 (1), pp.59-66. 〈10.1002/jobm.201500417〉
Journal of Basic Microbiology, Wiley-VCH Verlag, 2016, 56 (1), pp.59-66. ⟨10.1002/jobm.201500417⟩
Journal of Basic Microbiology, 2016, 56 (1), pp.59-66. ⟨10.1002/jobm.201500417⟩
ISSN: 0233-111X
Popis: International audience; Sco7697, a gene encoding a phytase, enzyme able to degrade phytate (myo-inositol 1,2,3,4,5,6-hexakis phosphate), the most abundant phosphorus storing compound in plants is present in the genome of S. coelicolor, a soil born bacteria with a saprophytic lifestyle. The expression of this gene was previously shown to be induced in conditions of Pi limitation by the response regulator PhoP binding to an operator sequence, the PHO box, located upstream of the -35 promoter sequence. A close examination of the promoter region of sco7697 revealed the presence of another putative operator site, a Direct Repeat (DR), located downstream of the -10 promoter sequence. In order to determine whether this DR played a role in regulation of sco7697 expression, different variants of the phytase gene promoter region were transcriptionally fused to the ß-glucuronidase reporter gene (GUS). As expected, deletion of the PHO box led to abolition of sco7697 induction in conditions of Pi limitation. Interestingly, alteration of the DR correlated with a dramatic increase of GUS expression but only when PhoP was present. These results demonstrated that this DR is the site of strong negative regulation by an unknown repressor. The latter would impede the necessary activation of phytase expression by PhoP.
Databáze: OpenAIRE
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