Ezrin enhances line tension along transcellular tunnel edges via NMIIa driven actomyosin cable formation
| Autor: | Stefani, Caroline, Gonzalez-Rodriguez, David, Senju, Yosuke, Doye, Anne, Efimova, Nadia, Janel, Sébastien, Lipuma, Justine, Tsai, Meng Chen, Hamaoui, Daniel, Maddugoda, Madhavi P., Cochet-Escartin, Olivier, Prévost, Coline, Lafont, Frank, Svitkina, Tatyana, Lappalainen, Pekka, Bassereau, Patricia, Lemichez, Emmanuel |
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| Přispěvatelé: | Institute of Biotechnology, Pekka Lappalainen / Principal Investigator, Centre méditerranéen de médecine moléculaire (C3M), Université Nice Sophia Antipolis (1965 - 2019) (UNS), COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Côte d'Azur (UCA), Université Nice Sophia Antipolis - Faculté de Médecine (UNS UFR Médecine), COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA), Laboratoire de Chimie et Physique - Approche Multi-échelle des Milieux Complexes (LCP-A2MC), Université de Lorraine (UL), Helsingin yliopisto = Helsingfors universitet = University of Helsinki, University of Pennsylvania, Centre d’Infection et d’Immunité de Lille - INSERM U 1019 - UMR 9017 - UMR 8204 (CIIL), Institut Pasteur de Lille, Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Lille-Centre Hospitalier Régional Universitaire [Lille] (CHRU Lille)-Centre National de la Recherche Scientifique (CNRS), Centre Hospitalier Régional Universitaire [Lille] (CHRU Lille), Sorbonne Université (SU), Laboratoire Physico-Chimie Curie [Institut Curie] (PCC), Institut Curie [Paris]-Institut de Chimie du CNRS (INC)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS), Université Nice Sophia Antipolis (... - 2019) (UNS), University of Helsinki, University of Pennsylvania [Philadelphia], Centre National de la Recherche Scientifique (CNRS)-Centre Hospitalier Régional Universitaire [Lille] (CHRU Lille)-Université de Lille-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut Pasteur de Lille, Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP), COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Université Côte d'Azur (UCA)-Institut National de la Santé et de la Recherche Médicale (INSERM) |
| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
DYNAMICS
F-ACTIN MIGRATION Nonmuscle Myosin Type IIA Science MYOSIN-II Actomyosin macromolecular substances STRESS FIBERS Article Actin Cytoskeleton Cytoskeletal Proteins PHENOTYPIC HETEROGENEITY EPITHELIAL CLOSURE CELLS Human Umbilical Vein Endothelial Cells Humans Surface Tension 1182 Biochemistry cell and molecular biology [SDV.BBM]Life Sciences [q-bio]/Biochemistry Molecular Biology ACTIN POLYMERIZATION STAPHYLOCOCCUS-AUREUS |
| Zdroj: | Nature Communications Nature Communications, 2017, 8, pp.15839. ⟨10.1038/ncomms15839⟩ Nature Communications, Vol 8, Iss 1, Pp 1-14 (2017) Nature Communications, Nature Publishing Group, 2017, 8, pp.15839. ⟨10.1038/ncomms15839⟩ |
| ISSN: | 2041-1723 |
| Popis: | Transendothelial cell macroaperture (TEM) tunnels control endothelium barrier function and are triggered by several toxins from pathogenic bacteria that provoke vascular leakage. Cellular dewetting theory predicted that a line tension of uncharacterized origin works at TEM boundaries to limit their widening. Here, by conducting high-resolution microscopy approaches we unveil the presence of an actomyosin cable encircling TEMs. We develop a theoretical cellular dewetting framework to interpret TEM physical parameters that are quantitatively determined by laser ablation experiments. This establishes the critical role of ezrin and non-muscle myosin II (NMII) in the progressive implementation of line tension. Mechanistically, fluorescence-recovery-after-photobleaching experiments point for the upstream role of ezrin in stabilizing actin filaments at the edges of TEMs, thereby favouring their crosslinking by NMIIa. Collectively, our findings ascribe to ezrin and NMIIa a critical function of enhancing line tension at the cell boundary surrounding the TEMs by promoting the formation of an actomyosin ring. Holes in endothelial barriers, called transendothelial cell macroapertures (TEMs), are predicted to be limited by line tension of unknown origin. Here the authors identify an actomyosin cable encircling TEMs and establish a role for ezrin in stabilising F-actin bundles, allowing their crosslinking by non-muscle myosin IIa. |
| Databáze: | OpenAIRE |
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