In Vitro Generation of Novel Functionalized Biomaterials for Use in Oral and Dental Regenerative Medicine Applications. Running Title: Fibrin–Agarose Functionalized Scaffolds
| Autor: | Cristina Blanco-Elices, Enrique España-Guerrero, Miguel Mateu-Sanz, David Sánchez-Porras, Óscar Darío García-García, María del Carmen Sánchez-Quevedo, Ricardo Fernández-Valadés, Miguel Alaminos, Miguel Ángel Martín-Piedra, Ingrid Garzón |
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| Přispěvatelé: | Universitat Politècnica de Catalunya. Doctorat en Enginyeria Biomèdica, Universitat Politècnica de Catalunya. BBT - Biomaterials, Biomecànica i Enginyeria de Teixits, Universidad de Granada |
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
extracellular matrix
oral and dental tissues Enginyeria dels materials [Àrees temàtiques de la UPC] lcsh:Technology Article Biomaterials Oral and dental tissues Tissue engineering lcsh:Microscopy Functionalization lcsh:QC120-168.85 lcsh:QH201-278.5 Implants dentals lcsh:T Dental implants Extracellular matrix Enginyeria de teixits lcsh:TA1-2040 Materials biomèdics tissue engineering Extracellular-matrix functionalization lcsh:Descriptive and experimental mechanics lcsh:Electrical engineering. Electronics. Nuclear engineering Erratum lcsh:Engineering (General). Civil engineering (General) lcsh:TK1-9971 Biomedical materials biomaterials |
| Zdroj: | Materials Volume 13 Issue 7 UPCommons. Portal del coneixement obert de la UPC Universitat Politècnica de Catalunya (UPC) Digibug. Repositorio Institucional de la Universidad de Granada instname Materials, Vol 13, Iss 1692, p 1692 (2020) |
| ISSN: | 1996-1944 |
| DOI: | 10.3390/ma13071692 |
| Popis: | Supplementary Materials The following are available online at https://www.mdpi.com/1996-1944/13/7/1692/s1, Table S1: Statistical comparison of staining intensity for histochemical and immunohistochemical analyses in the specific samples considered in the present study. Recent advances in tissue engineering offer innovative clinical alternatives in dentistry and regenerative medicine. Tissue engineering combines human cells with compatible biomaterials to induce tissue regeneration. Shortening the fabrication time of biomaterials used in tissue engineering will contribute to treatment improvement, and biomaterial functionalization can be exploited to enhance scaffold properties. In this work, we have tested an alternative biofabrication method by directly including human oral mucosa tissue explants within the biomaterial for the generation of human bioengineered mouth and dental tissues for use in tissue engineering. To achieve this, acellular fibrin–agarose scaffolds (AFAS), non-functionalized fibrin-agarose oral mucosa stroma substitutes (n-FAOM), and novel functionalized fibrin-agarose oral mucosa stroma substitutes (F-FAOM) were developed and analyzed after 1, 2, and 3 weeks of in vitro development to determine extracellular matrix components as compared to native oral mucosa controls by using histochemistry and immunohistochemistry. Results demonstrate that functionalization speeds up the biofabrication method and contributes to improve the biomimetic characteristics of the scaffold in terms of extracellular matrix components and reduce the time required for in vitro tissue development. Spanish Plan Nacional de Investigacion Cientifica, Desarrollo e Innovacion Tecnologica (I +D +I) of the Spanish Ministry of Economy and Competitiveness (Instituto de Salud Carlos III) (ERDF-FEDER, European Union) FIS PI18/331 PI18/332 Plan propio, Universidad de Granada PSETC_19_001 Consejeria de Economia, Conocimiento, Empresas y Universidad, Junta de Andalucia (European Regional Development Fund -FEDER) |
| Databáze: | OpenAIRE |
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