Increasing creatine kinase activity protects against hypoxia / reoxygenation injury but not against anthracycline toxicity in vitro
| Autor: | Zervou, S, Whittington, HJ, Ostrowski, PJ, Cao, F, Tyler, J, Lake, HA, Neubauer, S, Lygate, CA |
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| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
lcsh:Medicine
Apoptosis Bioenergetics Transfection Research and Analysis Methods Biochemistry Mice Open Reading Frames Gene Expression and Vector Techniques Medicine and Health Sciences Animals Humans Anthracyclines Cloning Molecular Molecular Biology Techniques lcsh:Science Creatine Kinase Molecular Biology Energy-Producing Organelles Staining Molecular Biology Assays and Analysis Techniques Base Sequence Cell Death Organic Compounds Organic Chemistry lcsh:R Chemical Compounds Biology and Life Sciences Cell Staining Heart Cell Biology Creatine Cell Hypoxia Mitochondria Isoenzymes Oxygen Chemistry HEK293 Cells Cytoprotection Doxorubicin Cell Processes Specimen Preparation and Treatment Physical Sciences Cardiovascular Anatomy Hyperexpression Techniques lcsh:Q Cellular Structures and Organelles Anatomy Research Article |
| Zdroj: | PLoS ONE, Vol 12, Iss 8, p e0182994 (2017) PLoS ONE |
| ISSN: | 1932-6203 |
| Popis: | The creatine kinase (CK) phosphagen system is fundamental to cellular energy homeostasis. Cardiomyocytes express three CK isoforms, namely the mitochondrial sarcomeric CKMT2 and the cytoplasmic CKM and CKB. We hypothesized that augmenting CK in vitro would preserve cell viability and function and sought to determine efficacy of the various isoforms. The open reading frame of each isoform was cloned into pcDNA3.1, followed by transfection and stable selection in human embryonic kidney cells (HEK293). CKMT2- CKM- and CKB-HEK293 cells had increased protein and total CK activity compared to non-transfected cells. Overexpressing any of the three CK isoforms reduced cell death in response to 18h hypoxia at 1% O2 followed by 2h re-oxygenation as assayed using propidium iodide: by 33% in CKMT2, 47% in CKM and 58% in CKB compared to non-transfected cells (P |
| Databáze: | OpenAIRE |
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