Differing requirements for the Arabidopsis Rad51 paralogs in meiosis and DNA repair

Autor: Bleuyard, Jean-Yves, Gallego, Maria, Savigny, Florence, White, Charles
Přispěvatelé: Génétique, Reproduction et Développement (GReD), Centre National de la Recherche Scientifique (CNRS)-Université Clermont Auvergne [2017-2020] (UCA [2017-2020])-Institut National de la Santé et de la Recherche Médicale (INSERM), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Clermont Auvergne [2017-2020] (UCA [2017-2020])-Centre National de la Recherche Scientifique (CNRS), White, Charles
Jazyk: angličtina
Rok vydání: 2016
Předmět:
animal structures
MESH: Mutation
DNA Repair
MESH: Sequence Homology
Amino Acid
Mitomycin
MESH: Cross-Linking Reagents
Molecular Sequence Data
Arabidopsis
[SDV.GEN] Life Sciences [q-bio]/Genetics
MESH: Amino Acid Sequence
MESH: Arabidopsis Proteins
MESH: Protein Isoforms
MESH: Phenotype
Protein Isoforms
MESH: Arabidopsis
MESH: Rad51 Recombinase
Amino Acid Sequence
MESH: Infertility
MESH: Phylogeny
Phylogeny
MESH: DNA Repair
[SDV.GEN]Life Sciences [q-bio]/Genetics
MESH: Molecular Sequence Data
Sequence Homology
Amino Acid
Arabidopsis Proteins
MESH: Mitomycin
fungi
MESH: Gamma Rays
DNA-Binding Proteins
Meiosis
enzymes and coenzymes (carbohydrates)
MESH: Meiosis
Cross-Linking Reagents
Phenotype
Gamma Rays
Infertility
Mutation
Rad51 Recombinase
biological phenomena
cell phenomena
and immunity
MESH: DNA-Binding Proteins
Zdroj: Plant Journal
Plant Journal, Wiley, 2005, 41 (4), pp.533-45. ⟨10.1111/j.1365-313X.2004.02318.x⟩
Plant Journal, 2005, 41 (4), pp.533-45. ⟨10.1111/j.1365-313X.2004.02318.x⟩
ISSN: 0960-7412
1365-313X
DOI: 10.1111/j.1365-313X.2004.02318.x⟩
Popis: International audience; In addition to the recombinase Rad51, vertebrates have five paralogs of Rad51, all members of the Rad51-dependent recombination pathway. These paralogs form two complexes (Rad51C/Xrcc3 and Rad51B/C/D/Xrcc2), which play roles in somatic recombination, DNA repair and chromosome stability. However, little is known of their possible involvement in meiosis, due to the inviability of the corresponding knockout mice. We have recently reported that the Arabidopsis homolog of one of these Rad51 paralogs (AtXrcc3) is involved in DNA repair and meiotic recombination and present here Arabidopsis lines carrying mutations in three other Rad51 paralogs (AtRad51B, AtRad51C and AtXrcc2). Disruption of any one of these paralogs confers hypersensitivity to the DNA cross-linking agent Mitomycin C, but not to gamma-irradiation. Moreover, the atrad51c-1 mutant is the only one of these to show meiotic defects similar to those of the atxrcc3 mutant, and thus only the Rad51C/Xrcc3 complex is required to achieve meiosis. These results support conservation of functions of the Rad51 paralogs between vertebrates and plants and differing requirements for the Rad51 paralogs in meiosis and DNA repair.
Databáze: OpenAIRE
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