DICER Regulates the Formation and Maintenance of Cell-Cell Junctions in the Mouse Seminiferous Epithelium
| Autor: | Korhonen, Hanna Maria, Yadav, Ram Prakash, Da Ros, Matteo, Chalmel, Frédéric, Zimmermann, Céline, Toppari, Jorma, Nef, Serge, Kotaja, Noora |
|---|---|
| Přispěvatelé: | University of Turku, Institut de recherche en santé, environnement et travail (Irset), Université d'Angers (UA)-Université de Rennes (UR)-École des Hautes Études en Santé Publique [EHESP] (EHESP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique ), Department of Genetic Medicine and Development [Geneva], Université de Genève = University of Geneva (UNIGE), Institute of Biomedicine/Physiology, Groupe d'Etude de la Reproduction Chez l'Homme et les Mammiferes (GERHM), Université de Rennes (UR)-Institut National de la Santé et de la Recherche Médicale (INSERM), Université d'Angers (UA)-Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-École des Hautes Études en Santé Publique [EHESP] (EHESP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique ), Université de Genève (UNIGE), Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-Institut National de la Santé et de la Recherche Médicale (INSERM) |
| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: |
Male
Ribonuclease III endocrine system Sertoli Cells blood-testis barrier urogenital system [SDV]Life Sciences [q-bio] cell-cell junction DICER Spermatids Up-Regulation DEAD-box RNA Helicases Mice Intercellular Junctions Seminiferous Epithelium Cell Adhesion Animals apical ectoplasmic specialization ddc:576.5 Claudin-5 gene regulation Spermatogenesis miRNA |
| Zdroj: | Biology of Reproduction Biology of Reproduction, 2015, 93 (6), pp.139. ⟨10.1095/biolreprod.115.131938⟩ Biology of Reproduction, Society for the Study of Reproduction, 2015, 93 (6), pp.139. ⟨10.1095/biolreprod.115.131938⟩ Biology of Reproduction, Vol. 93, No 6 (2015) P. 139 |
| ISSN: | 0006-3363 1529-7268 |
| Popis: | International audience; The endonuclease DICER that processes micro-RNAs and small interfering RNAs is essential for normal spermatogenesis and male fertility. We previously showed that the deletion of Dicer1 gene in postnatal spermatogonia in mice using Ngn3 promoter-driven Cre expression caused severe defects in the morphogenesis of haploid spermatid to mature spermatozoon, including problems in cell polarization and nuclear elongation. In this study, we further analyzed the same mouse model and revealed that absence of functional DICER in differentiating male germ cells induces disorganization of the cell-cell junctions in the seminiferous epithelium. We detected discontinuous and irregular apical ectoplasmic specializations between elongating spermatids and Sertoli cells. The defective anchoring of spermatids to Sertoli cells caused a premature release of spermatids into the lumen. Our findings may help also explain the abnormal elongation process of remaining spermatids because these junctions and the correct positioning of germ cells in the epithelium are critically important for the progression of spermiogenesis. Interestingly, cell adhesion-related genes were generally upregulated in Dicer1 knockout germ cells. Claudin 5 ( Cldn5 ) was among the most upregulated genes and we show that the polarized localization of CLAUDIN5 in the apical ectoplasmic specializations was lost in Dicer1 knockout spermatids. Our results suggest that DICER-dependent pathways control the formation and organization of cell-cell junctions in the seminiferous epithelium via the regulation of cell adhesion-related genes |
| Databáze: | OpenAIRE |
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