| Autor: |
Da, Mundus, Aa, Malygin, Ma, Zenkova, Mn, Repkova, Ag, Ven Iaminova, Sk, Smailov, Av, Li, Bulat Iskakov, Lv, Vratskikh, Vi, Iamkovoĭ |
| Předmět: |
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| Zdroj: |
Europe PubMed Central |
| Popis: |
Affinity labeling of 40S subunits from human placenta with 4-(N-2-chloroethyl-N-methylamino)benzylmethyl-[32P]phosphoamide s of oligoribonucleotides pAUG and pAUGU3 was studied. Covalent attachment of these derivatives to 40S subunits within the complexes with 40S subunits, formed in the presence of Met-tRNAf.eIF-2.GTP, was detected. Both rRNA and ribosomal proteins were modified. Fragments of 18S rRNA, containing sites of the reagent attachment were identified: 1058-1164 for pAUG derivative and 976-1057--for pAUG and pAUGU3 ones. The data obtained allowed to conclude that the presence of the neighbouring codon at the A-site, regardless of the presence of the tRNA in it, affects significantly the arrangement of the trinucleotide template in the codon-anticodon interaction region. The large subunit does not cause significant alterations in the structural organization of the codon-anticodon interaction region. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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