Phosphorylation of filamin A regulates chemokine receptor CCR2 recycling
| Autor: | Pons, Mònica, Izquierdo, Ismael, Andreu-Carbó, Mireia, Garrido, Georgina, Planagumà, Jesús, Muriel, Olivia, Pozo, Miguel A. del, Geli, María Isabel, Aragay, Anna M. |
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| Přispěvatelé: | Ministerio de Economía y Competitividad (España), Generalitat de Catalunya |
| Rok vydání: | 2016 |
| Předmět: |
Receptors
CCR2 Filamins Microfilament Proteins G-protein-coupled receptors Endosomes Actins Endocytosis GPCR CCR2 Filamin A Recycling Phosphoserine HEK293 Cells Membrane Microdomains Microscopy Fluorescence Gene Knockdown Techniques Humans Receptors Adrenergic beta-2 Phosphorylation HeLa Cells Signal Transduction |
| Zdroj: | Journal of Cell Science, vol. 130, no. 2, pp. 490-501 Digital.CSIC. Repositorio Institucional del CSIC instname |
| ISSN: | 1477-9137 |
| Popis: | Proper endosomal trafficking of ligand-activated G-protein-coupled receptors (GPCRs) is essential to spatiotemporally tune their physiological responses. For the monocyte chemoattractant receptor 2 (CCR2B; one of two isoforms encoded by CCR2), endocytic recycling is important to sustain monocyte migration, whereas filamin A (FLNa) is essential for CCL2-induced monocyte migration. Here, we analyze the role of FLNa in the trafficking of CCR2B along the endocytic pathway. In FLNa-knockdown cells, activated CCR2B accumulated in enlarged EEA-1-positive endosomes, which exhibited slow movement and fast fluorescence recovery, suggesting an imbalance between receptor entry and exit rates. Utilizing super-resolution microscopy, we observed that FLNa–GFP, CCR2B and β2-adrenergic receptor (β2AR) were present in actin-enriched endosomal microdomains. Depletion of FLNa decreased CCR2B association with these microdomains and concomitantly delayed CCR2B endosomal traffic, without apparently affecting the number of microdomains. Interestingly, CCR2B and β2AR signaling induced phosphorylation of FLNa at residue S2152, and this phosphorylation event was contributes to sustain receptor recycling. Thus, our data strongly suggest that CCR2B and β2AR signals to FLNa to stimulate its endocytosis and recycling to the plasma membrane This work was supported by grants from Ministerio de Economía y Competitividad ́ (BFU2011-30080 and BFU2014-53978-R); and Agència de Gestió d’Ajuts Universitaris i de Recerca (SGR41635). G.G. and I.I. were supported by Investigator Research Fellowships (Agéncia de Gestiód’Ajuts Universitaris i de Recerca). |
| Databáze: | OpenAIRE |
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