Functional genomics approaches to elucidate vulnerabilities of intrinsic and acquired chemotherapy resistance
| Autor: | Cetin, Ronay, Quandt, Eva, Kaulich, Manuel |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
RNAi and CRISPR screens
Antineoplastic Agents Review Vulnerabilidades al cáncer Vulnerabilitats del càncer Translational Research Biomedical Resistència a la quimioteràpia Quimioteràpia Humans Chemotherapy ddc:610 Resistencia a la quimioterapia Càncer Genòmica funcional lcsh:QH301-705.5 Cancer cancer and drug vulnerabilities Cancer vulnerabilities Functional genomics Genomics Cáncer Pantallas RNAi y CRISPR Genómica funcional lcsh:Biology (General) Pantalles RNAi i CRISPR Drug Resistance Neoplasm Gene Targeting Quimioterapia CRISPR-Cas Systems Chemotherapy resistance |
| Zdroj: | Cells Cells, Vol 10, Iss 260, p 260 (2021) |
| Popis: | Drug resistance is a commonly unavoidable consequence of cancer treatment that results in therapy failure and disease relapse. Intrinsic (pre-existing) or acquired resistance mechanisms can be drug-specific or be applicable to multiple drugs, resulting in multidrug resistance. The presence of drug resistance is, however, tightly coupled to changes in cellular homeostasis, which can lead to resistance-coupled vulnerabilities. Unbiased gene perturbations through RNAi and CRISPR technologies are invaluable tools to establish genotype-to-phenotype relationships at the genome scale. Moreover, their application to cancer cell lines can uncover new vulnerabilities that are associated with resistance mechanisms. Here, we discuss targeted and unbiased RNAi and CRISPR efforts in the discovery of drug resistance mechanisms by focusing on first-in-line chemotherapy and their enforced vulnerabilities, and we present a view forward on which measures should be taken to accelerate their clinical translation. info:eu-repo/semantics/publishedVersion |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|