| Autor: |
Ino, Hideo, Hayashi, Kazuhiko, Yanai, Hiroyuki, Teramoto, Norihiro, Koirala, Tirtha Raj, Chen, Hong-Li, Oka, Takashi, Yoshino, Tadashi, Takahashi, Kiyoshi, Akagi, Tadaastu |
| Rok vydání: |
1997 |
| Předmět: |
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| Zdroj: |
Acta medica Okayama. 51(4) |
| ISSN: |
0386-300X |
| Popis: |
A simian cell line, Si-IIA, harboring Epstein-Barr-virus (EBV) -related herpesvirus (Si-IIA-EBV), produces malignant lymphoma in rabbits when administered by intravenous inoculation. In this study, we analyzed the Si-IIA-EBV genome and compared it with human EBV and herpesvirus macaca fascicularis 1 (HVMF 1 ), which is associated with B-cell lymphoma developing in SIV-infected immunosuppressed monkeys. DNA from Si-IIA-EBV was amplified by the polymerase chain reaction using three different primer pairs complementary to human EBV (B95-8) DNA; two of the primer pairs covered part of the long internal repeat 1 region (IR 1) and the third covered part of the BRRF 1 region. Direct sequencing of the three PCR products revealed that Si-IIA-EBV DNA had about 82% nucleotide homology to the human EBV DNA in all three regions and 92.4% homology to HVMF1 in the IR1 region. The blotting pattern by Southern blot analysis was different between Si-IIA-EBV and human EBV. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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