Prueba de difusión con discos de oxacilina para predecir la resistencia a la penicilina de Streptococcus pneumoniae
| Autor: | Horna G, Ml, Molero, Benites L, Roman S, Carbajal L, Mercado E, Me, Castillo, Rito Zerpa, Chaparro E, Hernandez R, Silva W, Campos F, Saenz A, Reyes I, Villalobos A, Tj, Ochoa |
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| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
lcsh:Arctic medicine. Tropical medicine
purl.org/pe-repo/ocde/ford#3.03.05 [https] lcsh:RC955-962 Penicillin Resistance lcsh:Public aspects of medicine Ceftriaxone lcsh:R lcsh:Medicine lcsh:RA1-1270 oxacillin Anti-Bacterial Agents Cross-Sectional Studies Latin America Streptococcus pneumoniae Disk Diffusion Antimicrobial Tests Peru Humans |
| Zdroj: | Revista Panamericana de Salud Pública, Vol 40, Iss 1, Pp 57-63 Scopus-Elsevier Europe PubMed Central |
| Popis: | Objective To 1) describe the correlation between the zones of inhibition in 1-µg oxacillin disk diffusion (ODD) tests and penicillin and ceftriaxone minimum inhibitory concentrations (MICs) of meningeal and non-meningeal strains of Streptococcus pneumoniae and 2) evaluate the usefulness of the ODD test as a predictor of susceptibility to penicillin in S. pneumoniae and as a quick and cost-effective method easily implemented in a routine clinical laboratory setting. Methods S. pneumoniae isolates from healthy nasopharyngeal carriers less than 2 years old, obtained in a multicentric cross-sectional study conducted in various Peruvian hospitals and health centers from 2007 to 2009, were analyzed. Using Clinical and Laboratory Standards Institute (CLSI) breakpoints, the correlation between the zones of inhibition of the ODD test and the MICs of penicillin and ceftriaxone was determined. Results Of the 571 S. pneumoniae isolates, 314 (55%) showed resistance to penicillin (MIC ≥ 0.12 µg/mL) and 124 (21.7%) showed resistance to ceftriaxone (MIC ≥ 1 µg/mL). Comparison of the ODD test zones of inhibition and the penicillin MICs, using the CLSI meningeal breakpoints, showed good correlation (Cohen’s kappa coefficient = 0.8239). Conclusions There was good correlation between ODD zones of inhibition and penicillin meningeal breakpoints but weak correlation between the ODD results and non-meningeal breakpoints for both penicillin and ceftriaxone. Therefore, the ODD test appears to be a useful tool for predicting penicillin resistance in cases of meningeal strains of S. pneumoniae, particularly in low- and middle- income countries, where MIC determination is not routinely available. |
| Databáze: | OpenAIRE |
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