Structural and electronic determinants of lytic polysaccharide monooxygenase reactivity on polysaccharide substrates

Autor: Simmons, T. J., Frandsen, K. E. H., Ciano, L., Tryfona, T., Lenfant, N., Poulsen, J. C., Wilson, L. F. L., Tandrup, T., Tovborg, M., Schnorr, K., Johansen, K. S., Henrissat, B., Walton, P. H., Lo Leggio, L, Dupree, P.
Přispěvatelé: Architecture et fonction des macromolécules biologiques (AFMB), Institut National de la Recherche Agronomique (INRA)-Aix Marseille Université (AMU)-Centre National de la Recherche Scientifique (CNRS), Centre National de la Recherche Scientifique (CNRS)-Aix Marseille Université (AMU)-Institut National de la Recherche Agronomique (INRA), UK Biotechnology and Biological Sciences Research Council BB/L000423/1 BB/L021633/1, Danish Council for Strategic Research 12-134923 12-134922, European Project: 283570,EC:FP7:INFRA,FP7-INFRASTRUCTURES-2011-1,BIOSTRUCT-X(2011)
Jazyk: angličtina
Rok vydání: 2017
Předmět:
Models
Molecular
MESH: Sordariales
Polysaccharides/chemistry
[SDV.BIO]Life Sciences [q-bio]/Biotechnology
[SDV.NEU.NB]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]/Neurobiology
Sordariales
MESH: Catalytic Domain
Mixed Function Oxygenases/chemistry
[SDV.IMM.II]Life Sciences [q-bio]/Immunology/Innate immunity
Mixed Function Oxygenases
Substrate Specificity
Polyporaceae
enzyme lytique
[SDV.MHEP.MI]Life Sciences [q-bio]/Human health and pathology/Infectious diseases
Catalytic Domain
lcsh:Science
[SDV.MHEP.ME]Life Sciences [q-bio]/Human health and pathology/Emerging diseases
Vegetal Biology
[SDV.BBM.BS]Life Sciences [q-bio]/Biochemistry
Molecular Biology/Structural Biology [q-bio.BM]
Polyporaceae/enzymology
monooxygénase
MESH: Mixed Function Oxygenases
[SDV.BIBS]Life Sciences [q-bio]/Quantitative Methods [q-bio.QM]
[SDV.BBM.BC]Life Sciences [q-bio]/Biochemistry
Molecular Biology/Biomolecules [q-bio.BM]
MESH: Copper
glucomannane
[SDV.BBM.BS]Life Sciences [q-bio]/Biochemistry
Molecular Biology/Biomolecules [q-bio.BM]
xyloglucane
[SDV.MP.VIR]Life Sciences [q-bio]/Microbiology and Parasitology/Virology
Copper/chemistry
MESH: Fungal Proteins
MESH: Models
Molecular
Fungal Proteins/chemistry
Science
Article
Fungal Proteins
MESH: Polyporaceae
Polysaccharides
[SDV.BBM.BC]Life Sciences [q-bio]/Biochemistry
Molecular Biology/Biochemistry [q-bio.BM]
Electron Spin Resonance Spectroscopy
[SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology
Sordariales/enzymology
carbohydrates (lipids)
MESH: Polysaccharides
polysaccharide
[SDV.SP.PHARMA]Life Sciences [q-bio]/Pharmaceutical sciences/Pharmacology
MESH: Electron Spin Resonance Spectroscopy
lcsh:Q
MESH: Substrate Specificity
Biologie végétale
Copper
Zdroj: Nature Communications
Nature Communications, Nature Publishing Group, 2017, 8 (1), pp.1064. ⟨10.1038/s41467-017-01247-3⟩
Nature Communications, Vol 8, Iss 1, Pp 1-12 (2017)
Simmons, T J, Frandsen, K E H, Ciano, L, Tryfona, T, Lenfant, N, Poulsen, J C, Wilson, L F L, Tandrup, T, Tovborg, M, Schnorr, K, Johansen, K S, Henrissat, B, Walton, P H, Lo Leggio, L & Dupree, P 2017, ' Structural and electronic determinants of lytic polysaccharide monooxygenase reactivity on polysaccharide substrates ', Nature Communications, vol. 8, 1064 . https://doi.org/10.1038/s41467-017-01247-3
Nature Communications, 2017, 8 (1), pp.1064. ⟨10.1038/s41467-017-01247-3⟩
Nature Communications (8), . (2017)
'Nature Communications ', vol: 8, pages: 1064-1-1064-12 (2017)
Simmons, T J, Frandsen, K E H, Ciano, L, Tryfona, T, Lenfant, N, Poulsen, J-C N, Wilson, L F L, Tandrup, T, Tovborg, M, Schnorr, K, Johansen, K S, Henrissat, B, Walton, P H, Lo Leggio, L & Dupree, P 2017, ' Structural and electronic determinants of lytic polysaccharide monooxygenase reactivity on polysaccharide substrates ', Nature Communications, vol. 8 . https://doi.org/10.1038/s41467-017-01247-3
ISSN: 2041-1723
DOI: 10.1038/s41467-017-01247-3⟩
Popis: Lytic polysaccharide monooxygenases (LPMOs) are industrially important copper-dependent enzymes that oxidatively cleave polysaccharides. Here we present a functional and structural characterization of two closely related AA9-family LPMOs from Lentinus similis (LsAA9A) and Collariella virescens (CvAA9A). LsAA9A and CvAA9A cleave a range of polysaccharides, including cellulose, xyloglucan, mixed-linkage glucan and glucomannan. LsAA9A additionally cleaves isolated xylan substrates. The structures of CvAA9A and of LsAA9A bound to cellulosic and non-cellulosic oligosaccharides provide insight into the molecular determinants of their specificity. Spectroscopic measurements reveal differences in copper co-ordination upon the binding of xylan and glucans. LsAA9A activity is less sensitive to the reducing agent potential when cleaving xylan, suggesting that distinct catalytic mechanisms exist for xylan and glucan cleavage. Overall, these data show that AA9 LPMOs can display different apparent substrate specificities dependent upon both productive protein–carbohydrate interactions across a binding surface and also electronic considerations at the copper active site.
Copper-dependent lytic polysaccharide monooxygenases (LPMOs) oxidatively cleave polysaccharides. Here the authors present a structure-function characterization of fungal LPMOs, showing that a particular LPMO cleaves xylan using a mechanism that involves an alternative copper coordination geometry.
Databáze: OpenAIRE
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