Docking of acetyl-CoA carboxylase to the plastid envelope membrane attenuates fatty acid production in plants
| Autor: | Ye, Yajin, Nikovics, Krisztina, To, Alexandra, Lepiniec, Loïc, Fedosejevs, Eric T., Van Doren, Steven R., Baud, Sébastien, Thelen, Jay J. |
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| Přispěvatelé: | University of Missouri [Columbia] (Mizzou), University of Missouri System, Institut Jean-Pierre Bourgin (IJPB), AgroParisTech-Université Paris-Saclay-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), National Science Foundation (grants MCB-1716688 and PGRP IOS-1829365, ANR-11-BTBR-0004,RAPSODYN,Optimisation de la teneur et du rendement en huile chez le colza cultivé sous contrainte azotée(2011), ANR-17-EURE-0007,SPS-GSR,Ecole Universitaire de Recherche de Sciences des Plantes de Paris-Saclay(2017) |
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Light
Science Arabidopsis Acetates Article Protein Domains Light responses [SDV.BBM]Life Sciences [q-bio]/Biochemistry Molecular Biology Carbon Radioisotopes Plastids Promoter Regions Genetic Arabidopsis Proteins Protoplasts Fatty Acids fungi Gene Expression Regulation Developmental food and beverages Intracellular Membranes Molecular Docking Simulation Protein Subunits Biofuels Plant biotechnology Secondary metabolism Acetyl-CoA Carboxylase Protein Binding |
| Zdroj: | Nature Communications Nature Communications, Nature Publishing Group, 2020, 11 (1), ⟨10.1038/s41467-020-20014-5⟩ Nature Communications, Vol 11, Iss 1, Pp 1-14 (2020) |
| ISSN: | 2041-1723 |
| DOI: | 10.1038/s41467-020-20014-5⟩ |
| Popis: | In plants, light-dependent activation of de novo fatty acid synthesis (FAS) is partially mediated by acetyl-CoA carboxylase (ACCase), the first committed step for this pathway. However, it is not fully understood how plants control light-dependent FAS regulation to meet the cellular demand for acyl chains. We report here the identification of a gene family encoding for three small plastidial proteins of the envelope membrane that interact with the α-carboxyltransferase (α-CT) subunit of ACCase and participate in an original mechanism restraining FAS in the light. Light enhances the interaction between carboxyltransferase interactors (CTIs) and α-CT, which in turn attenuates carbon flux into FAS. Knockouts for CTI exhibit higher rates of FAS and marked increase in absolute triacylglycerol levels in leaves, more than 4-fold higher than in wild-type plants. Furthermore, WRINKLED1, a master transcriptional regulator of FAS, positively regulates CTI1 expression by direct binding to its promoter. This study reveals that in addition to light-dependent activation, “envelope docking” of ACCase permits fine-tuning of fatty acid supply during the plant life cycle. In plants, light-dependent activation fatty acid synthesis (FAS) is mediated in part by acetyl-CoA carboxylase (ACCase). Here the authors identify a family of genes encoding carboxyltransferase interactors that attenuate FAS in the light by docking acetyl-CoA carboxylase to the plastid envelope. |
| Databáze: | OpenAIRE |
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