Double-band sarcomeric SHG pattern induced by adult skeletal muscles alteration during myofibrils preparation
| Autor: | Recher, Gaëlle, Rouède, Denis, Tascon, Christophe, D'Amico, Laure-Anne, Tiaho, François |
|---|---|
| Přispěvatelé: | Interactions cellulaires et moléculaires (ICM), Université de Rennes (UR)-Centre National de la Recherche Scientifique (CNRS), Institut de Physique de Rennes (IPR), Région Bretagne, Rennes Métropole, Conseil Général d′Ille-et-Vilaine and the Ministère de l'enseignement supérieur et de la recherche, Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-Centre National de la Recherche Scientifique (CNRS) |
| Jazyk: | angličtina |
| Rok vydání: | 2011 |
| Předmět: |
Sarcomeres
MESH: TISSUE MESH: 2ND-HARMONIC GENERATION MESH: Tissue Preservation MESH: MYOCYTES Models Biological Muscle proteolysis Specimen Handling MESH: Myofibrils Xenopus laevis MESH: Xenopus laevis MESH: FILAMENTS Animals MESH: Microscopy Confocal second harmonic generation microscopy MESH: Animals skeletal muscle MESH: Specimen Handling Muscle Skeletal myofibrils MESH: Muscle Skeletal MESH: MYOSIN Microscopy Confocal MESH: Sarcomeres MESH: Models Biological [SDV.BBM.BM]Life Sciences [q-bio]/Biochemistry Molecular Biology/Molecular biology Tissue Preservation MESH: ENDOGENOUS STRUCTURAL PROTEINS MESH: MICROSCOPY MESH: COLLAGEN |
| Zdroj: | Journal of Microscopy Journal of Microscopy, 2011, 241 (2), pp.207-11. ⟨10.1111/j.1365-2818.2010.03425.x⟩ Journal of Microscopy, Wiley, 2011, 241 (2), pp.207-11. ⟨10.1111/j.1365-2818.2010.03425.x⟩ |
| ISSN: | 0022-2720 1365-2818 |
| DOI: | 10.1111/j.1365-2818.2010.03425.x⟩ |
| Popis: | International audience; To understand the reported difference between double band, sarcomeric second harmonic generation pattern of isolated myofibril and predominant single band pattern found in thick muscle tissues, we studied the effect of myofibril preparation on the second harmonic generation pattern. We found that double band sarcomeric second harmonic generation pattern usually observed in myofibrils (prepared from fresh tissue) is due to muscle alteration during the mixing and triton treatment processes. Single band sarcomeric second harmonic generation pattern could be observed in isolated myofibrils when this alteration is previously prevented using paraformaldehyd fixed tissue. We conclude that single band sarcomeric second harmonic generation pattern is a signature of adult muscle myofibrils in normal physiological condition, suggesting that sarcomeric second harmonic generation patterns could be used as a valuable diagnosis tool of muscle health. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Plný text